way to dispose of a small amount of liquid acrylamide is to polymerize it in the hood in a closed plastic bag set into a beaker surrounded by a very large, tightly fastened plastic bag, to prevent attering as the acrylamide polymerizes If you have more than 100ml to dispose of, contact your local environmental safety officers to determine your recommended procedure. Acrylamide solutions emit significant heat during polymerization, and polymerization of large volumes of acryla mide can be explosive due to rapid heat buildup( dow Chemical Company, 1988; Cytec Industries, 1995; Bio-Rad Laboratories, 2000) Acrylamide and bis-acrylamide powders must be disposed of as solid hazardous waste. Consult your local environmental safety What Is the Shelf Life of Acrylamide and Acrylamide Solutions? Commercially prepared acrylamide solutions are stable for as g as one year, unopened, and for six months after opening. The igh purity of the solution components and careful monitoring throughout the manufacturing process provides extended shelf life. The lifetime of homemade solutions similarly depends on the purity of the acrylamide and bis-acrylamide, the cleanliness of the laboratory dishes, and the purity of the water used to make he solutions a Solid acrylamide breaks down with time due to oxidation and TV light, producing acrylic acid and ammonia. Acrylic acid in a gel can cause fuzzy bands, or fuzzy spots in the case of 2-D gels, streaking and smearing, and poor resolution(Allen and Budowle 1994) Acrylamide decomposition occurs more quickly in solution, and it can be accelerated by any impurities within the water(Allen and Budowle, 1994). Thus acrylamide powder should be stored airtight at room temperature, and acrylamide solutions should be stored at 4C. both in the dark Production facilities must establish standards and measures to determine the effective lifetime of unpolymerized acrylamide ELECTRICAL SAFETY What Are the Requirements for a safe Work Area? The voltages used in electrophoresis can be dangerous, fires have occurred due to problems with electrophoresis cell 336 Boozway to dispose of a small amount of liquid acrylamide is to polymerize it in the hood in a closed plastic bag set into a beaker surrounded by a very large, tightly fastened plastic bag, to prevent spattering as the acrylamide polymerizes. If you have more than 100ml to dispose of, contact your local environmental safety officers to determine your recommended procedure. Acrylamide solutions emit significant heat during polymerization, and polymerization of large volumes of acryla￾mide can be explosive due to rapid heat buildup (Dow Chemical Company, 1988; Cytec Industries, 1995; Bio-Rad Laboratories, 2000). Acrylamide and bis-acrylamide powders must be disposed of as solid hazardous waste. Consult your local environmental safety office. What Is the Shelf Life of Acrylamide and Acrylamide Solutions? Commercially prepared acrylamide solutions are stable for as long as one year, unopened, and for six months after opening. The high purity of the solution components and careful monitoring throughout the manufacturing process provides extended shelf life. The lifetime of homemade solutions similarly depends on the purity of the acrylamide and bis-acrylamide, the cleanliness of the laboratory dishes, and the purity of the water used to make the solutions. Solid acrylamide breaks down with time due to oxidation and UV light, producing acrylic acid and ammonia. Acrylic acid in a gel can cause fuzzy bands, or fuzzy spots in the case of 2-D gels, streaking and smearing, and poor resolution (Allen and Budowle, 1994).Acrylamide decomposition occurs more quickly in solution, and it can be accelerated by any impurities within the water (Allen and Budowle, 1994). Thus acrylamide powder should be stored airtight at room temperature, and acrylamide solutions should be stored at 4°C, both in the dark. Production facilities must establish standards and measures to determine the effective lifetime of unpolymerized acrylamide solutions. ELECTRICAL SAFETY What Are the Requirements for a Safe Work Area? The voltages used in electrophoresis can be dangerous, and fires have occurred due to problems with electrophoresis cells.The 336 Booz