cDNA第二链的合成 自身引导法:获得的双链cDNA5’端会有几对碱基缺 失 G 5 ppp G AAAAAAAAAAAAAAOH 3 TTTTTTTTTTTTTTp 5 NaoH 煮沸 哩 TTTTTTTTp5 AoH 3 Klenow dNTPs TTTTTTTTTTTTTTp 5 AAAAAAAAAAAAAAOH 3 1 AAAAAAAAAAAAAAcDNA第二链的合成 NaOH 煮沸 自身引导法：获得的双链cDNA 5’端会有几对碱基缺 失 AAAAAAAAAAAAAA G 5‘ppp’5G AAAAAAAAAAAAAAOH 3’ TTTTTTTTTTTTTTp 5’ TTTTTTTTTTTTTTp 5’ TTTTTTTTTTTTTTp 5’ AAAAAAAAAAAAAAOH 3’ TTTTTTTTTTTTTT OH 3’ Klenow dNTPs S1