cDNA第二链的合成 置换合成法:获得的双链cDNA5’端也会有几对碱基缺 G 5 pppG AAAAAAAAAAAAAAOH 3 四中中TTTp5 RNaesh DNApol dNTPs AAAATTTOH 3 TTTTTTTTTTTTTTp 5 TTTTTTTTTTTTTTOH 3 AAAAAAAAAAAAAAp5′ S1 T4-DNA ligase 53 TTTTTTTTTTTTTT 3 AAAAAAAAAAAAAA 5cDNA第二链的合成 RNaesH DNApol dNTPs 置换合成法：获得的双链cDNA 5’端也会有几对碱基缺 失G 5‘ppp’5G AAAAAAAAAAAAAAOH 3’ TTTTTTTTTTTTTTp 5’ AAAAAAAAAAAAAAp 5’ 5’ S1 AAAATTTOH 3’ TTTTTTTTTTTTTTp 5’ 5’ TTTTTTTTTTTTTTOH 3’ AAAAAAAAAAAAAA 5’ 5’ TTTTTTTTTTTTTT 3’ 3’ T4-DNA ligase