基因工程原理实验指导 Adjust to pH 8.0 ddH2O to 1000ml sterilize by autoclaving 1 MTris-HCl pH7.4 pH7.6 pH8.0 Tris base 121.1g 121.1g 121.1g Concentracted HCl 70 ml 64 ml 42 ml ddH2O to 1000ml 1000ml 1000ml Sterilize by autoclaving TE (pH8.0) Stock vol. 10 mM Tris-HCI pH 8.0) IM 10 ml 1 mMEDTA (DH 80) 0.5M 2 ml ddH2O to 1000ml sterilize by autoclaving 10 MNH4Ac NH.Ac 385g 770g H2O to 500ml 1000ml 10×PCR buffer stock 500 mM KCI 2.5 M(sterilized) 200ml 100 mM Tris-HCI 1 M pH 9.0(sterilized)100 ml 1%Triton X-100 100% 10 ml ddH2O 690ml sterilize by autoclaving 5×TBE Tris 54g Boric acid 27.5g 0.5 M EDTA pH 7.9) 20 ml ddH2Oto 1000ml 10×TAE Tris 121.1g 484.4g EDTA(0.5 M) 20 ml 80 ml NaAc:3H2O 17g 68g glacial acetic acid 30m1 200ml基因工程原理实验指导 23 Adjust to pH 8.0 ddH2O to 1000 ml sterilize by autoclaving 1 M Tris·HCl pH 7.4 pH 7.6 pH 8.0 Tris base 121.1 g 121.1 g 121.1 g Concentracted HCl 70 ml 64 ml 42 ml ddH2O to 1000 ml 1000 ml 1000 ml Sterilize by autoclaving TE ( pH 8.0) Stock vol. 10 mM Tris·HCl ( pH 8.0) 1 M 10 ml 1 mM EDTA ( pH 8.0) 0.5 M 2 ml ddH2O to 1000 ml sterilize by autoclaving 10 M NH 4 Ac NH４Ac 385 g 770 g H2O to 500 ml 1000 ml 10 × PCR buffer stock vol. 500 mM KCl 2.5 M(sterilized) 200 ml 100 mM Tris-HCl 1 M pH 9.0 (sterilized) 100 ml 1% Triton X-100 100% 10 ml ddH2O 690 ml sterilize by autoclaving 5 × TBE Tris 54 g Boric acid 27.5 g 0.5 M EDTA ( pH 7.9) 20 ml ddH2O to 1000 ml 10 × TAE Tris 121.1 g 484.4 g EDTA(0.5 M) 20 ml 80 ml NaAc·3H2O 17 g 68 g glacial acetic acid 30 ml 200 ml