基因工程原理实验指导 Amp（100mg/ml) 1gAmp溶于4ml去离子灭菌双蒸水中，定容至5ml,-20℃保存 附录二质粒抽提试剂 Solution】 Cell resuspension solution(50 mM Tris-HCI,pH 7.5,10 mM EDTA,RNase A 100 ug/ml) 1 M Tris-HCl(pH 7.6) 2.5ml 0.25 MEDTA 2.0ml ddH2O 45 ml sterile by autoclave add 1%RNase 0.5ml store at room temperature SolutionⅡ Cell lysis solution (0.2 N NaOH,1%SDS) Mix 0.4 N NaOH and 2%SDS in same volume SolutionⅢ Neutralization solution(1.32 M potassium acetate.pH 5.2) 5M potassium acetate 13.2ml ddH2O 27ml adjust pH to 5.2 add ddH2O to 50ml sterile by autoclave store at room temperature 附录三DNA操作试剂 1.5×CTAB CTAB 15g 1 M Tris·C1(pH8.0) 75 ml 0.5 MEDTA 30ml NaCl 61.4g add ddH2O to 1000ml 0.5 M EDTA pH 8.0) EDTA-Na-2H2O 186.1g NaOH 20g 22 基因工程原理实验指导 22 Amp （ 100mg/ml ）： 1g Amp 溶于 4ml 去离子灭菌双蒸水中，定容至 5ml ， -20 ℃保存 附录二 质粒抽提试剂 Solution Ι Cell resuspension solution (50 mM Tris-HCl, pH 7.5, 10 mM EDTA, RNase A 100 µg/ml) 1 M Tris-HCl (pH 7.6) 2.5 ml 0.25 M EDTA 2.0 ml ddH2O 45 ml sterile by autoclave add 1% RNase 0.5ml store at room temperature Solution Ⅱ Cell lysis solution (0.2 N NaOH, 1% SDS) Mix 0.4 N NaOH and 2% SDS in same volume. Solution Ⅲ Neutralization solution (1.32 M potassium acetate, pH 5.2) 5 M potassium acetate 13.2 ml ddH2O 27 ml adjust pH to 5.2 add ddH2O to 50 ml sterile by autoclave store at room temperature 附录三 DNA 操作试剂 1.5 × CTAB CTAB 15 g 1 M Tris · Cl (pH 8.0) 75 ml 0.5 M EDTA 30 ml NaCl 61.4 g add ddH2O to 1000 ml 0.5 M EDTA ( pH 8.0) EDTA-Na·2H2O 186.1 g NaOH 20 g