上游充通大￥ SHANGHAI JIAO TONG UNIVERSITY FLASH 革兰氏染色过程 1、用碱性染料结晶紫 Stop 1 Flood the heat-foxed 对菌液涂片进行初染 Sproad culture in thin smear with crystal fim over slido violet for 1 minute All cells purple 2、用碘液进行媒染。 Stop 2 Add lodine solution for 3 minutes 3、用乙醇冲洗进行脱 All cells remain purple 色。 Step 3 Pass slide through flame to fix Decolorze with alcohol briefly about 20 seconds 4、用碱性染料沙黄进 Gram-positive cels are purple: Gram-negative colls 行复染。革兰氏阴性 are colorless 细菌最后呈现桃红到 Flood slide with stain; rinse and dry Step4 红色，而革兰氏阳性 Counton倍Lain with safranin for 1 100X to 2 minutes 细菌保持深紫色 Gram-positive cells are purplo: Placo drop of oil on slide: Gram-nogative cella 5 examino with 100 x objectivo are red Staining colls for microscoplc observation.5 上海交通大学通识教育核心课程《微生物的世界》 革兰氏染色过程 1、用碱性染料结晶紫 对菌液涂片进行初染 2、用碘液进行媒染。 3、用乙醇冲洗进行脱 色。 4、用碱性染料沙黄进 行复染。革兰氏阴性 细菌最后呈现桃红到 红色，而革兰氏阳性 细菌保持深紫色 FLASH