532 XU Yu-chao et al.Journal of Integrative Agriculture 2016,15(3):528-536 Brassica rapa ssp.pekinensis Brassica rapa ssp.chinensis sica napus Eutrma salaugineum Capsella nbella Camelina sativa Arabidopsis lyrata subsp.lyrata Arabidopsis 33323388F Tarenaya hasslenana mnsps:r Brassica rapa ssp.pekinensis Brassica rapa ssp.chinensis Bra ssica napus Eutrma sala Camelina sativa Arabidopsis lyrata subsp.lyrata Arabidopsis thaliana 4464331455591454 Tarenaya hasslerian egg kirkr nkkng Brassica rapa ssp.pekinensis Brassica rapa ssp.chinensis ssica napu Eutrma salaugineum Capsella rbella Camelina sativa Arabidopsis lyrata subsp.lyrata Arabidopsis thaliana Tarenaya hassleriana arayd Brassica rapa ssp.pekinensis Brassica rapa ssp.chinensis Eutrma salaugineum CaDs8a几bea Camelina sativa Arabidopsis lyrata subsp.lyrata Arabidopsis thaliana 0招新新 Tarenaya hasslenana Fig.4 Alignment of the conserved ERF signatures of BrcERF-B3 with other ERF-B3 from different plants.The identical amino acid residues were indicated with black background,while 75%conservation was marked as gray.The deduced motifs(3 B-sheets and 1 a-helix)were marked. 98 Capsella rubella Camlina sativa 100 Arabidopsis lyrata subsp.lyrata Arabidopsis thaliana Eutrema salsugineum 100 Brassica napus 95 Brassica rapa ssp.pekinensis Brassica rapa ssp.chinensis Tarenaya hassleriana Fig.5 The homology tree was conducted to show the relationships of ERF-B3 among different plants. 3.Discussion the nucleotide sequences of two BrcERF-B3 genes were identical between these two lines.The phylogenetic tree Non-heading Chinese cabbage is an important crop in revealed that BrcERF-B3 belonged to the ERF-B3 subfamily. eastern Asia.Stamen-petalody is a novel type of male which is similar to the result study that most of 77 encoding sterility in non-heading Chinese cabbage.In this study,we ERF-like proteins of ERF superfamily belonging to B3 in rice cloned the full-ORF cDNA sequences of BrcERF-B3 from (Sharoni et al.2011).The DBD of BrcERF-B3 protein,which two non-heading Chinese cabbage lines and discovered that contained 3 anti-parallel B-sheet and 1 a-helix,combined532 XU Yu-chao et al. Journal of Integrative Agriculture 2016, 15(3): 528–536 3. Discussion Non-heading Chinese cabbage is an important crop in eastern Asia. Stamen-petalody is a novel type of male sterility in non-heading Chinese cabbage. In this study, we cloned the full-ORF cDNA sequences of BrcERF-B3 from two non-heading Chinese cabbage lines and discovered that the nucleotide sequences of two BrcERF-B3 genes were identical between these two lines. The phylogenetic tree revealed that BrcERF-B3 belonged to the ERF-B3 subfamily, which is similar to the result study that most of 77 encoding ERF-like proteins of ERF superfamily belonging to B3 in rice (Sharoni et al. 2011). The DBD of BrcERF-B3 protein, which contained 3 anti-parallel β-sheet and 1 α-helix, combined Fig. 4 Alignment of the conserved ERF signatures of BrcERF-B3 with other ERF-B3 from different plants. The identical amino acid residues were indicated with black background, while 75% conservation was marked as gray. The deduced motifs (3 β-sheets and 1 α-helix) were marked. Fig. 5 The homology tree was conducted to show the relationships of ERF-B3 among different plants