Pesticide Analytical Manual Vol. I APPENDⅨ APPENDIX II: PROTOCOLS AND REPORTING FORMS FOR TESTING CHEMICALS THROUGH PAMI MULTIRESIDUE METHODS INTRODUCTION: MULTIRESIDUE METHOD TESTING Use of any multiresidue method(MRM)is supported by available information about how potential residues behave through the steps of the method To provide that support for PAM I MRMS, additional chemicals are continually tested through the method steps and the resulting data compiled in a single database. All PAM I tables in Chapters 3 and 4 and Appendix I are produced from that database This Appendix provides directions for performing such tests and forms for report- ing results The effort spent on the testing of MRMs and compilation of results is justified by the advantages such compilations offer the analytical chemist. When analytical behavior data for numerous chemicals through the method in use are known, the analyst is better equipped to identify residues that may be present in a sample of unknown treatment history. In situations where the likelihood of some particular residue is known, the data lists for several methods can be consulted to help choose which method should be used Regulatory agencies often must assess the incidence of residue occurrence. This effort is also assisted by compilations of method behavior data. The absence of many chemicals from the sample can be ascertained when it is known that those chemicals could have been detected had they been present It has been found advisable to define protocols for developing data on MRM behavior. In order to compile data into usable formats, it is imperative that all contributing laboratories perform the tests uniformly. The goal of this method- testing is not to find the optimum conditions for the one chemical currently being tested, but to be able to describe how the chemical will behave when determined by the precisely defined method This Appendix includes one protocol for determining GlC characteristics of chemi- cals and six protocols for testing their behavior through individual MRMs. Forms for reporting the results of testing by each protocol are also included. Each pro- tocol references the PAM I method(s) involved, the types of chemicals to which applies, and the PAM I table(s) in which previously collected data are published Some PAM I MRMs are applicable to a wide variety of residues, while others are targeted to those with specific chemical structures. A Decision Tree is included in this Appendix to direct the user to the most appropriate protocol(s) for each chemical being tested. Follow the Decision Tree in deciding which protocol(s)to Follow the steps of these protocols, in the order written Report data on a copy of the appropriate form and send it to: PAM I Editors, HFS-337, Food and Drug Administration, 200 C Street SW, Washington, DC 20204 ppendⅸ‖1Pesticide Analytical Manual Vol. I APPENDIX II Transmittal No. 94-1 (1/94) Form FDA 2905a (6/92) Appendix II–1 APPENDIX II: PROTOCOLS AND REPORTING FORMS FOR TESTING CHEMICALS THROUGH PAM I MULTIRESIDUE METHODS INTRODUCTION: MULTIRESIDUE METHOD TESTING Use of any multiresidue method (MRM) is supported by available information about how potential residues behave through the steps of the method. To provide that support for PAM I MRMs, additional chemicals are continually tested through the method steps and the resulting data compiled in a single database. All PAM I tables in Chapters 3 and 4 and Appendix I are produced from that database. This Appendix provides directions for performing such tests and forms for report￾ing results. The effort spent on the testing of MRMs and compilation of results is justified by the advantages such compilations offer the analytical chemist. When analytical behavior data for numerous chemicals through the method in use are known, the analyst is better equipped to identify residues that may be present in a sample of unknown treatment history. In situations where the likelihood of some particular residue is known, the data lists for several methods can be consulted to help choose which method should be used. Regulatory agencies often must assess the incidence of residue occurrence. This effort is also assisted by compilations of method behavior data. The absence of many chemicals from the sample can be ascertained when it is known that those chemicals could have been detected had they been present. It has been found advisable to define protocols for developing data on MRM behavior. In order to compile data into usable formats, it is imperative that all contributing laboratories perform the tests uniformly. The goal of this method￾testing is not to find the optimum conditions for the one chemical currently being tested, but to be able to describe how the chemical will behave when determined by the precisely defined method. This Appendix includes one protocol for determining GLC characteristics of chemi￾cals and six protocols for testing their behavior through individual MRMs. Forms for reporting the results of testing by each protocol are also included. Each pro￾tocol references the PAM I method(s) involved, the types of chemicals to which it applies, and the PAM I table(s) in which previously collected data are published. Some PAM I MRMs are applicable to a wide variety of residues, while others are targeted to those with specific chemical structures. A Decision Tree is included in this Appendix to direct the user to the most appropriate protocol(s) for each chemical being tested. Follow the Decision Tree in deciding which protocol(s) to use. Follow the steps of these protocols, in the order written. Report data on a copy of the appropriate form and send it to: PAM I Editors, HFS-337, Food and Drug Administration, 200 C Street SW, Washington, DC 20204