APPENDIX‖l Pesticide Analytical Manual Vol I Decision Tree for MRM Testing Do data already exist (Index to Methods, Appendix I]? evelop only data nd t Does the compound have this structure or characteristic Yes N-methylcarbamate? Test per Protocol A Yes naturally fluorescent? Test per Protocol A. acid or phenol? Yes Test per Protocol B Yes substituted urea? Test per Protocol G Does it chromatograph on GLC systems? vel I Determine GLC characteristics per Protocol C, Level I Yes Discontinue tests Does it chromatograph on at least one Level system in a reasonable time [o3<rrt<5.0)? No Report rrts for Level I DG modules tested, then retest using appropriate Level ll DG module[s] Is the product a nonfatty (27 fat] food? Yesh Test per Protocol F for fatty food Test per Protocol D. Is the chemical recovered through Section 302 extraction? No Yesh Discontinue tests Test per protocol e for nonfatty food ppendⅸ|2 vo.941(1 2905a(6Transmittal No. 94-1 (1/94) Appendix II–2 Form FDA 2905a (6/92) APPENDIX II Pesticide Analytical Manual Vol. I Decision Tree for MRM Testing Do data already exist (Index to Methods, Appendix I)? Develop only data not yet available. Yes No Does the compound have this structure or characteristic: Yes Yes Yes Yes Determine GLC characteristics per Protocol C, Level I. Does it chromatograph on GLC systems? No Discontinue tests. No Yes Does it chromatograph on at least one Level I system in a reasonable time (0.3<rrt<5.0)? No Yes Report rrts for Level I DG modules tested, then retest using appropriate Level II DG module(s). Is the product a nonfatty (2% fat) food? No Yes Test per Protocol F for fatty food. Test per Protocol D. Is the chemical recovered through Section 302 extraction? No Yes Discontinue tests. Test per Protocol E for nonfatty food. N-methylcarbamate? naturally fluorescent? acid or phenol? substituted urea? Test per Protocol A. Test per Protocol A. Test per Protocol B. Test per Protocol G