www.nature.com/scientificreports/ Predicted function of the core microbiota and non-core microbiota in vinegar Pei.To vali hich and funct For this as a separat a The tw dopapad ompared and visu xcel and Origin (v.8.0) References son of microbial c nity of different typical Chines ria in th 5.Xu,W.et al.Mo ty du lid-state ation of zhe ted food.Appl.Enviro 177,2264-227 d ci,P.Caggia,C.Bacterial po uated by L M tem gene expression during spo s wheat and spelt tions.En (2011 tionnt..Food 139,284-2922010 Reid.G.Bacterial metatranscriptome analysis of a probiotic yogurt using an RNA-Seg nd its n SME5187-183(201 16,283293(202 in mic 200N M.cta PeeaLAda1os.2472222o data. on in the fermentation of G.c ctic resonance spectroscop 19 y.Lee.s. during fer tion of saeu-jeot so prepare nships w 52(201 24 2g6S6190 2010 ie.2.O.e (201 ure vinega wang.M.D 28 of rice vi r (Komesu)and et al.Application o add bacteria in 31 o.M acetic acid b inerar It.I Foo on of rice v sessed by nous yeast population from traditional balsamic impact on flavor Agr 30072 M. iullo.M.C 12192(006) SCIENTIFIC REPORTS6:26818DOl:10.1038/srep26818www.nature.com/scientificreports/ Scientific Reports | 6:26818 | DOI: 10.1038/srep26818 9 Predicted function of the core microbiota and non-core microbiota in vinegar Pei. To vali￾date the function of the core microbiota for the whole community in vinegar Pei, phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt), was used to predict which gene families were present48. Given that the copy number and function of bacteria were more abundant than fungi, PICRUSt was performed base on the bacterial 16S gene surveys. For this analysis, OTUs were closed-reference picked against the Greengenes by QIIME (v.1.7). The functional core taxonomies were filtered as a separate dataset of core microbiota while the remaining taxonomies were regarded as another dataset of non-core microbiota. The two datasets were normalised, predicted, and categorised according to online protocols of PICRUSt (http://hutten￾hower.sph.harvard.edu/galaxy). The predicted functions of the core microbiota and non-core microbiota were compared and visualised in Microsoft® Excel and Origin (v.8.0). References 1. Hugenholtz, J. 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