PCR Annealing primers primers bind to the template sequence Taq polymerase recognizes double-stranded substrate 5 3 ↓ Primers anneal at 55ocAnnealing Primers •Primers bind to the template sequence •Taq polymerase recognizes double-stranded substrate 3′ 5′ 5′ 3′ Primers anneal at 55oC 3′ 5′ 5′ 3′ 3′ 5′ 5′ 3′ PCR 28