PCR Tag polymerase extends ●●6●● Tag polymerase extends primer DNA is replicated 5 3 Extend at 72oC 国国国国国国回回回回回国国国国圆_■■■■□ 3~ Repeat denaturing, annealing, and extending 30 cycles5′ 3′ 3′ Taq polymerase extends...... 3′ 5′ 5′ Extend at 72oC 5′ 3′ •Taq polymerase extends primer •DNA is replicated Repeat denaturing, annealing, and extending 30 cycles 5′ 3′ 5′ 3′ 3′ 5′ 5′ 3′ PCR 29