RT-PCR AAAAAAAAA 3 mRNA reverse transcriptase anchor primer (VTTTT )V=G, C or A AAAAAAAAA 3 mRNA 3 TTTT CDNA RNase or RNase activity of reverse transcriptase TTTT 5′cDMA Taq DNA polymerase sequence-specific primer(-) anchor primer ds cdNA TTTT 5′ 5 3 5RT-PCR 30