BEH. 462/3.962J Molecular Principles of Biomaterials Spring 2003 sialyl lewis x receptor Therapeutic cargo IL-1B TNF Site of intibody-based targeting Targeted Activation local activation of a conjugate by action of enzymes or cellular environment example of cathepsin-sensitive linkages 'Reverse'targeting attraction of target cells to carrier Attraction of target cells to device via chemotaxis Dendritic ce∥s -1 per 100 cells in most tissues BSA rod BSA rod BSA rod MIP-3B/BSA rod+ BSA rod F BSA rod +OVA rodt b. MIP-3B/BSA rod+ OVA rod Tissue sections stained for MHc class ll(expressed by antigen-presenting cells) ( Kumamoto et al. 2002) Lecture 17-Drug targeting 5 of 7BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Lecture 17 – Drug targeting 5 of 7 Site of inflammation IL-1β TNF sialyl lewisX receptor Therapeutic cargo ƒ antibody-based targeting Targeted Activation ƒ local activation of a conjugate by action of enzymes or cellular environment ƒ example of cathepsin-sensitive linkages ‘Reverse’ targeting6 ƒ attraction of target cells to carrier Attraction of target cells to device via chemotaxis: (Kumamoto et al. 2002) Tissue sections stained for MHC class II (expressed by antigen-presenting cells) Dendritic cells ~1 per 100 cells in most tissues