CONTENTS Page INTR0D0CTI0N… METHODS AND MATERIALS ................................................. 3 Hatchery Sampling 3 Sampling for Physiology........................................... plasma Electrolytes ......................................... 4 Gi11Wa-K'ATPase… Disease Sampling.................................................. Life History of Hatchery Juveniles .......................... 5 Blood Sample collection...................................... 5 Viral assays................................................. Histopathology… Bacteriological Assays....... 】 RESULTS AND DISCUSSION OF HATCHERY STEELHEAD SURVEYS................... 9 Chelan Hatchery (Transferred to Leavenworth Hatchery)Steelhead... Gill Nat-8t arease ................................................ plasma Electrolytes.......................................... 9 Hematology.................................................. Viral screening................................17 Indirect Fluorescent Antibody Test for Bacterial Kidney Disease...............................17 Histopathology···························· 17 Seawater AdaptationCONTENTS Page INTRODUCTION ........................................................... METHODS AND MATERIALS .................................................. Hatchery Sampling ................................................. Sampling for Physiology ........................................... Plasma Electrolytes .......................................... Gill Na+ -K+ ATPase ........................................... Disease Sampling .................................................. Life History of Hatchery Juveniles ........................... Blood Sample Collection ...................................... Viral Assays ................................................. Histopathology ............................................... Bacteriological Assays ....................................... RESULTS AND DISCUSSION OF HATCHERY STEELHEAD SURVEYS ................... Chelan Hatchery (Transferred to Leavenworth Hatchery) Steelhead ... Gill Na+-K+ ATPase ........................................... Plasma Electrolytes .......................................... Hematology ................................................... 1 3 3 4 4 4 5 5 5 7 7 7 9 9 9 9 13 Viral Screening . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Indirect Fluorescent Antibody Test for Bacterial Kidney Disease . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Histopathology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Seawater Adaptation . . . . . . . . . . . . . . . . . . . . . . . . . . . 19