scroll far enough, you will see the enzyme lysozyme listed. Returning to the former screen and clicking on Structure Neighbors will display about eight structures similar to human alpha-lactalbumin. Note again the presence of lysozyme in the list. Clicking on"Other Sources"will display other data files with references to alpha-lactalbumin. It is interesting to note that the proteins alpha-lactalbumin and lysozyme have similar primary, secondary, and tertiary structures but they have quite different biochemical activities. The two proteins, which have about 40% sequence identity, may have been derived from a common ancestral Another useful structure tool is RasMol (or Ras Mac). This will allow you to view the detailed structure of a protein and rotate it on coordinates so you can see it from all perspectives. A hyperlink to Ras Mol is present under the"View Structure"function just above "Chime". You may need to study RasMol instructions provided under Help, or you may use a RasMol tutorial listed in Table El. 2. Another useful protein viewer is the Swiss-Protein Pdv viewer (Table E1. 2). BLAST is an advanced sequence similarity tool availableatNcbl.Toaccessthisgotothencbihomepage(www.ncbinlm.nihgov)and click on“ BLAST”. Then click on“ Basic blast search” to obtain a dialogue box into which you may type the amino acid sequence of human alpha-lactalbumin. This process may be streamlined by downloading the amino acid sequence in FASTA format into a file and transferring the file into the blast dialogue box. BLAST will provide a list of proteins with equences similar to the one entered Another approach to a study of protein (or nucleic acid) structure and sequences is through Entrez. This can be entered via the NCBI home page. Then click onProteins"to obtain a dialogue box where you can type human alpha-lactalbumin"and then click on Search. You can retrieve about 25 documents for review. Note that you may also enter BLaST through entrez. On the web Introduction to the biochemistry Laboratory http://www.graphpad.com/prism/prism.htm Software for statistics and curve fitting. http://www.statistics.com Click on Free Web-based software for data analysis.304 scroll far enough, you will see the enzyme lysozyme listed. Returning to the former screen and clicking on Structure Neighbors will display about eight structures similar to human alpha-lactalbumin. Note again the presence of lysozyme in the list. Clicking on “Other Sources” will display other data files with references to alpha-lactalbumin. It is interesting to note that the proteins alpha-lactalbumin and lysozyme have similar primary, secondary, and tertiary structures but they have quite different biochemical activities. The two proteins, which have about 40% sequence identity, may have been derived from a common ancestral gene. Another useful structure tool is RasMol (or RasMac). This will allow you to view the detailed structure of a protein and rotate it on coordinates so you can see it from all perspectives. A hyperlink to RasMol is present under the “View Structure” function just above “Chime”. You may need to study RasMol instructions provided under Help, or you may use a RasMol tutorial listed in Table E1.2. Another useful protein viewer is the Swiss-Protein Pdv Viewer (Table E1.2). BLAST is an advanced sequence similarity tool available at NCBI. To access this, go to the NCBI home page (www.ncbi.nlm.nih.gov) and click on “BLAST”. Then click on “Basic BLAST search” to obtain a dialogue box into which you may type the amino acid sequence of human alpha-lactalbumin. This process may be streamlined by downloading the amino acid sequence in FASTA format into a file and transferring the file into the BLAST dialogue box. BLAST will provide a list of proteins with sequences similar to the one entered. Another approach to a study of protein (or nucleic acid) structure and sequences is through Entrez. This can be entered via the NCBI home page. Then click on “Proteins” to obtain a dialogue box where you can type “human alpha-lactalbumin” and then click on Search. You can retrieve about 25 documents for review. Note that you may also enter BLAST through Entrez. On the Web Introduction to the Biochemistry Laboratory http://www.graphpad.com/prism/Prism.htm Software for statistics and curve fitting. http://www.statistics.com/ Click on Free Web-based software for data analysis