3. If Anfinsen had not determined the location of the s-s bonds in renatured rnasea his result of >90% recovery of enzyme activity would a have proven his hypothesis b not have been believed c not have been possible d have been hard to believe e have gone unnoticed 4. The statement(from Campbell, p 127)that"the amino acid sequence of a protein contains all of the information required for the complete three dimensional structure a cannot be falsified by experiment; hence it is trivial B is strongly supported by denaturation-renaturation experiments C is called an"hypothesis"since its valid ity is sort of dicey D is called a"model"because it can be diagrammed in textbooks e has essentially been proven by recent theoretical work 5. Anfinsen probably chose RNase A for his experiments instead of say, trypsin or chymotrypsin, because a trypsin would digest itself to oligopeptides under mildly denaturing. conditions(slow removal of urea). b RNase A is less expensive than trypsin or chymotrypsin C Trypsin and chymotrypsin are intracellular enzymes(no S-s bonds) d RNase a has only 25 amino acid residues e RNase a does not have any interfering His residues 6. Which of the following occurred when RNase A refolded? a The primary structure of the protein was rearranged b Most of the charged residues were found buried in the protein C The entropy of the protein in d All of the above e None of ※<7> 1. Cleavage of an IgG molecule by the protease, papain, produces a an antigen -bind ing site and two constant regions B two heavy chain-light chain dimers C an inactive mixture of oligopeptides d two Fab fragments and one Fc fragment E an antibody without its N-linked oligosaccharide 2. Antibodies of the Igg class3. If Anfinsen had not determined the location of the S-S bonds in renatured RNaseA, his result of >90% recovery of enzyme activity would A have proven his hypothesis. B not have been believed. C not have been possible. D have been hard to believe. E have gone unnoticed. 4. The statement (from Campbell, p.127) that "the amino acid sequence of a protein contains all of the information required for the complete three dimensional structure" A cannot be falsified by experiment; hence it is trivial. B is strongly supported by many denaturation-renaturation experiments. C is called an "hypothesis" since its validity is sort of dicey. D is called a "model" because it can be diagrammed in textbooks. E has essentially been proven by recent theoretical work. 5. Anfinsen probably chose RNaseA for his experiments instead of say, trypsin or chymotrypsin, because A trypsin would digest itself to oligopeptides under mildly denaturing conditions (slow removal of urea). B RNaseA is less expensive than trypsin or chymotrypsin. C Trypsin and chymotrypsin are intracellular enzymes (no S-S bonds). D RNaseA has only 25 amino acid residues. E RNaseA does not have any interfering His residues. 6. Which of the following occurred when RNaseA refolded? A The primary structure of the protein was rearranged. B Most of the charged residues were found buried in the protein. C The entropy of the protein increased. D All of the above. E None of the above. 5 ※<7> 1. Cleavage of an IgG molecule by the protease, papain, produces: A an antigen-binding site and two constant regions. B two heavy chain-light chain dimers. C an inactive mixture of oligopeptides. D two Fab fragments and one Fc fragment. E an antibody without its N-linked oligosaccharide. 2. Antibodies of the IgG class