Name ection: Question 2 A tRNa molecule(shown below)is composed of an Rna chain that folds into a 3-D shape like that shown below. At one end it has an anti-codon that base pairs with the appropriate codon on the mRna and at the other end it has an amino acid arm that binds to a specific amino acid Below are three anti-codon sequences for three tRNAs, fill in the corresponding amino acid on the blanks Amino acid arm Anticodon Figure by MIT OCW anticodon found on trna amino acid attached to trNA SAGU 3 5 5 b) You are studying a certain enzyme. You have two mutant versions, one that works just as well as the wild-type and another that doesn't work at all. When you sequence the genes that code for these two mutant enzymes you find the enzyme that works has a 9 base pair deletion, whereas the enzyme that does not work has only a single base pair deletion. Explain why the sequence with the 9 base pair deletion results in an active enzyme when the sequence with a single base pair deletion produces an inactive enzyme cThe first 17 nucleotides from an mRNa molecule are GAaUGGCaCUUagCaa Write out the first 5 amino acids encoded by this sequence 7012Fall2003_____________________ _____________________ _____________________ Name:___________________________________ Section:_____ Question 2 A tRNA molecule (shown below) is composed of an RNA chain that folds into a 3-D shape like that shown below. At one end it has an anti-codon that base pairs with the appropriate codon on the mRNA and at the other end it has an amino acid arm that binds to a specific amino acid. Below are three anti-codon sequences for three tRNAs, fill in the corresponding amino acid on the blanks. Amino acid arm Anticodon Amino acid arm Anticodon Figure by MIT OCW. anticodon found on tRNA amino acid attached to tRNA 5’ AGU 3’ 5’ AUG 3’ 5’ CUG 3’ b) You are studying a certain enzyme. You have two mutant versions, one that works just as well as the wild-type and another that doesn’t work at all. When you sequence the genes that code for these two mutant enzymes you find the enzyme that works has a 9 base pair deletion, whereas the enzyme that does not work has only a single base pair deletion. Explain why the sequence with the 9 base pair deletion results in an active enzyme when the sequence with a single base pair deletion produces an inactive enzyme. c)The first 17 nucleotides from an mRNA molecule are 5’ GAAUGGCCACUUAGCAA…3’ Write out the first 5 amino acids encoded by this sequence. 7.012 Fall 2003 2