MIT Biology Department 7.012: Introductory Biology-Fall 2004 Instructors: Professor Eric Lander, Professor Robert A. Weinberg Dr Claudette Gardel Name Section 7.012 Problem set 4 Please print out this problem set and record your answers on the printed copy. Answers to this problem set are to be turned in at the box outside by 4: 10 Wednesday, October 8. Problem sets will not be accepted late Solutions will be posted on the web October 9, 2003. Question 1 Below is an electron micrograph of a single gene being transcribed. The DNA strand runs horizontally with RNa transcripts extending vertically outward Image removed due to copyright reasons. a)Draw an arrow indicating the direction that the Rna polymerases are transcribing.Why did you choose this direction? b) Below is a partial sequence of the above gene. Its orientation is the same as pictured above Which strand is the template strand the top or the bottom strand? Explain your choice ACTCGATGCTAG S TGAGCTACGATC c)What would be the mRNA sequence transcribed from the above sequence? Be sure to label the5′and3′ends 7012Fall2003MIT Biology Department 7.012: Introductory Biology - Fall 2004 Instructors: Professor Eric Lander, Professor Robert A. Weinberg, Dr. Claudette Gardel Name:___________________________________ Section:_____ 7.012 Problem Set 4 Please print out this problem set and record your answers on the printed copy. Answers to this problem set are to be turned in at the box outside by 4:10 Wednesday, October 8. Problem sets will not be accepted late. Solutions will be posted on the web October 9, 2003. Question 1 Below is an electron micrograph of a single gene being transcribed. The DNA strand runs horizontally with RNA transcripts extending vertically outward. Image removed due to copyright reasons. a) Draw an arrow indicating the direction that the RNA polymerases are transcribing. Why did you choose this direction? b) Below is a partial sequence of the above gene. Its orientation is the same as pictured above. Which strand is the template strand, the top or the bottom strand? Explain your choice. 5’ACTCGATGCTAG3’ 3’TGAGCTACGATC5’ c) What would be the mRNA sequence transcribed from the above sequence? Be sure to label the 5’ and 3’ ends. 7.012 Fall 2003 1