Thiamin Determination in Foods 5 g enriched flour 75 ml 0.1 N HCl Make volume to 100 ml Digest at 1000C for 30 min Centrifuge and filterThiamin Determination in Foods 5 g enriched flour 75 ml 0.1 N HCl Make volume to 100 ml Digest at 1000C for 30 min Centrifuge and filter