THIAMIN DETERMINATION THIOCHROME (Fluorescent) N K3 Fe(CN)6 Oxidation N S N H3C N CH2 CH3 CH2CH2OH N S N H3C N CH2 NH2 CH3 CH2CH2OH pyrimidine thiazole Excite thiochrome at 365 nm and measure the absorbance at 435 nmTHIAMIN DETERMINATION THIOCHROME (Fluorescent) N K3 Fe(CN)6 Oxidation N S N H3C N CH2 CH3 CH2CH2OH N S N H3C N CH2 NH2 CH3 CH2CH2OH pyrimidine thiazole Excite thiochrome at 365 nm and measure the absorbance at 435 nm