11. Which one of the following determine the structure o c. have no effect a. Transmission electron microscope b. the above 16.适于观察细胞表面及断面超微结构三维图 c. Differential centr ifugatioin d像的仪器是() X-ray crystallography a.普通光镜b.荧光显微镜c.相差光 None of the above 镜d.扫描电镜ε.透射电镜 17.红细胞中O2与OO2交换作用主要依靠 12. You stain membrane proteins with a a.血影蛋白b.血型糖蛋白 Ⅲ蛋 fluorescent-labeled antibody and notice the 白d.锚定蛋白e.肌动蛋白 membrane is even ly stained. After a few18.能与胞外信号特异识别和结合,介导胞内 hours. all of the fluorescence is at one end 信使生成,引起细胞产生效应的是() of a cell. you can conclude tha A. carrier protein B. channel protein ceptor D Proteins move laterally in the membrane 19.下列连接方式中属于与中间纤维相连的锚 The fluorescent dye was bleached.d定连接的是() Proteins act as transporters a.粘合带;b.粘合斑 桥粒 13. You have a preparation of ghosts and need20.带电的离子之所以不能自由通过质膜其 to collect only the right-side out ghosts. You 原因是() decide you can trap the right-side out ghosts 膜的孔径不够大 in an affinity column packed with( b.膜的电性与离子电性相斥; c带电的离子聚集成团 carbohydrate-binding molecule d带电离子通常与水形成水性外壳以至 c. a protein-binding antibody 于体积过大 四、简答题(毎题4分,选做5题,共20分) 14. All of following ind ividuals1.基因敲除 knockout实验中有哪些关键环节 ntributed to cell theory except( 影响试验的成败? a. Robert Hooke c. Theodor Schwann 2.真核生物与原核生物的rRNA基因在组织 15. The addition of a peptide with an RGD结构、转录和加工中有什么 sequence would probably the binding 差异? of cultured cells to a fibronectin coated4 11. Which one of the following was used to determine the structure of the DNA molecule?( ) a. Transmission electron microscope b. Scanning electron microscope c. Differential centrifugatioin d. X-ray crystallography e. None of the above 12. You stain membrane proteins with a fluorescent-labeled antibody and notice the membrane is evenly stained. After a few hours, all of the fluorescence is at one end of a cell. You can conclude that( ). a. Lipids flip-flop in the membrane. b. Proteins move laterally in the membrane. c. The fluorescent dye was bleached. d. Proteins act as transporters. e. All of the above 13. You have a preparation of ghosts and need to collect only the right-side out ghosts. You decide you can trap the right-side out ghosts in an affinity column packed with( ) a. SDS. b. A carbohydrate-binding molecule. c. A protein-binding antibody. d. A serine-binding molecule. e. None of the above 14. All of the following individuals contributed to cell theory except( ) a. Robert Hooke. b. Matthias Schleiden. c. Theodor Schwann. d. Rudolf Virchow. 15. The addition of a peptide with an RGD sequence would probably ? the binding of cultured cells to a fibronectin coated dish.( ) a. enhance b. inhibit c. have no effect on d. none of the above 16. 适于观察细胞表面及断面超微结构三维图 像的仪器是( ) a．普通光镜 b．荧光显微镜 c．相差光 镜 d．扫描电镜 e．透射电镜 17. 红细胞中 O2 与 CO2 交换作用主要依靠 ( ) a．血影蛋白 b．血型糖蛋白 c. 带Ⅲ蛋 白 d．锚定蛋白 e. 肌动蛋白 18. 能与胞外信号特异识别和结合，介导胞内 信使生成，引起细胞产生效应的是( ) A ． carrier protein B ．channel protein C．receptor D．1iand E．enzyme 19. 下列连接方式中属于与中间纤维相连的锚 定连接的是( )。 a．粘合带； b．粘合斑； c．桥粒； d．紧密连接。 20. 带电的离子之所以不能自由通过质膜,其 原因是( ) a.膜的孔径不够大; b.膜的电性与离子电性相斥; c.带电的离子聚集成团; d.带电离子通常与水形成水性外壳,以至 于体积过大。 四、简答题(每题 4 分， 选做 5 题，共 20 分) 1. 基因敲除(knockout)实验中有哪些关键环节 影响试验的成败？ 2. 真核生物与原核生物的 rRNA 基因在组织 结构、转录和加工中有什么 差异？