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第2期 曹成波等：新型胶原支架材料的结构特征与性能 197. xenogenic dermal mat rix implantation in immunocompetent rats. 型胶原复合膜的制备及性能研究.中国医学物理学杂志， JSurg Res,.2001,96(1):96 2003,20(4):293 [11】姜笃银，陈壁，贾赤宇，等.异种脱细胞真皮基质抗原性的实 16]蒋维棋，皮革成品理化检验北京：中国轻工业出版社， 验研究.中华烧伤杂志，2003,19(3)：155 1999,82 [12]刘德伍，李国辉，邹萍，等.表皮细胞、成纤维细胞复合脱细胞 [17]Chen R N.Ho HO.Tsai Y T,et al.Process development of an 真皮基质构建组织工程皮肤.中国临床康复，2004,8(8)： acellular dermal matrix (ADM)for biomedical applications.Bio- 1439 materials,2004,25,2679 [13]曹成波，吴克安.一种胶原基复合支架材料及其制备方法和用 [18]Queen D.Evans J H.Gaylor J DS,et al.An in vitro assessment 途：中国专利，CN03139063.3.20040908 of wound dressing conformability.Biomaterials.1987.8:372. [14]肖莉莎.Ch4s/胶原杂化活性人工皮肤的研究[学位论文] [19]付小兵，王德文，创伤修复基础.北京：人民军医出版社，1997： 广州：暨南大学，2000：16 282 [15]黄汉萍，牟善松，屠美，等.活性人工皮肤的骨架一一种新 Properties and structure characterization of new type collagen scaffold materials CAO Chengbo),LV Ronghui,ZHANG Chunlian2,ZOU Yuping,SONG Guodong,ZHANG Changgiao 1)Chemistry and Chemical Engineering School,Shandong University,Jinan 250100,China 2)Shandong University Hospital.Jinan 250061,China 3)Jinan Central Hospital.Jinan 250013.China ABSTRACI In order to overcome the shortcomings of acellular dermal matrix used as tissue engineering scaf- fold material,such as bad permeability,low degradation speed,and strong immunogenicity,a new type of colla- gen scaffold was prepared by applying various chemical,biological and physical comprehensive methods to deal with pigskin.The microstructure of the scaffold was observed under photomicroscope and scanning electron mi- croscopy (SEM),and the in vitro degradation time,permeability,tensile-strength,porosity,shrinkage temper- ature were tested.The experimental results show that its dermal fibroblasts,lipocytes and interstitial substances between collagen fibers have been removed completely,simultaneously collagen fibers have been appropriately loosen,and the original natural three-dimensional meshwork porous structure is maintained;the permeability is about 3000g'md which is fit for wound healing:the degradation time is between 25h and 50h and can be controlled by adjusting technological conditions:the tensile-strength is between 10.20 MPa and 11.50 MPa which is better;the shrinkage temperature is between 70C and 85C.All of those indicate that the material overcomes the shortcomings of acellular dermal matrix and has high permeability,tensile"strength,and proper degradation speed.So it conforms to tissue engineering scaffold material requirements. KEY WORDS collagen;scaffold material;structure characterization;propertiesxenogenic dermal matrix implantation in immunocompetent rats． J Surg Res200196（1）：96 ［11］ 姜笃银陈壁贾赤宇等．异种脱细胞真皮基质抗原性的实 验研究．中华烧伤杂志200319（3）：155 ［12］ 刘德伍李国辉邹萍等．表皮细胞、成纤维细胞复合脱细胞 真皮基质构建组织工程皮肤．中国临床康复20048（8）： 1439 ［13］ 曹成波吴克安．一种胶原基复合支架材料及其制备方法和用 途：中国专利CN03139063．3．2004－09－08 ［14］ 肖莉莎．Ch4S／胶原杂化活性人工皮肤的研究 ［学位论文 ］． 广州：暨南大学2000：16 ［15］ 黄汉萍牟善松屠美等．活性人工皮肤的骨架－－－一种新 型胶原复合膜的制备及性能研究．中国医学物理学杂志 200320（4）：293 ［16］ 蒋维祺．皮革成品理化检验．北京：中国轻工业出版社 1999：82 ［17］ Chen R NHo H OTsai Y Tet al．Process development of an acellular dermal matrix （ADM） for biomedical applications．Biomaterials200425：2679 ［18］ Queen DEvans J HGaylor J D Set al．An in vitro assessment of wound dressing conformability．Biomaterials19878：372． ［19］ 付小兵王德文．创伤修复基础．北京：人民军医出版社1997： 282 Properties and structure characterization of new type collagen scaffold materials CAO Chengbo 1） LV Ronghui 1） ZHA NG Chunlian 2） ZOU Y uping 1） SONG Guodong 3） ZHA NG Changqiao 1） 1） Chemistry and Chemical Engineering SchoolShandong UniversityJinan250100China 2） Shandong University HospitalJinan250061China 3） Jinan Central HospitalJinan250013China ABSTRACT In order to overcome the shortcomings of acellular dermal matrix used as tissue engineering scaffold materialsuch as bad permeabilitylow degradation speedand strong immunogenicitya new type of collagen scaffold was prepared by applying various chemicalbiological and physical comprehensive methods to deal with pigskin．The microstructure of the scaffold was observed under photomicroscope and scanning electron microscopy （SEM）and the in vitro degradation timepermeabilitytensile-strengthporosityshrinkage temperature were tested．The experimental results show that its dermal fibroblastslipocytes and interstitial substances between collagen fibers have been removed completelysimultaneously collagen fibers have been appropriately loosenand the original natural three-dimensional meshwork porous structure is maintained；the permeability is about 3000g·m －2·d －1which is fit for wound healing；the degradation time is between25h and50h and can be controlled by adjusting technological conditions；the tensile-strength is between10∙20MPa and11∙50MPa which is better；the shrinkage temperature is between70℃ and85℃．All of those indicate that the material overcomes the shortcomings of acellular dermal matrix and has high permeabilitytensile-strengthand proper degradation speed．So it conforms to tissue engineering scaffold material requirements． KEY WORDS collagen；scaffold material；structure characterization；properties 第2期 曹成波等： 新型胶原支架材料的结构特征与性能 ·197·