EcoR I ↓ 5'..GAATTC...3'→ 5..G AATTC...3' 3...CTTAAG...5' 3'...CTTAA G..5 Pst I ↓ 5'..CTGCAG..3'→ 5'...CTGCA G...3 3'...GACGTC...5' 3..G ACGTC...5' Sma I ↓ 5..CCCGGG...3→ 5...CCC GGG...3' 3'...GGGCCC...5' 3'...GGG CCC...5