TABLE 4.4 Examples of culture media for microorganisms with simple and demanding nutritional requirements Defined culture medium for Defined culture medium for Complex culture medium for Escherichia coli Leuconostoc mesenteroides either E. coli or L. mesenteroides K2HPO4 7g K2HPO4 0.6 g Glucose 15 KH2PO: 2 g KH POA 0.6 Yeast extract 5 Peptone 5 g (NHD2SO: 1 g NHC 3 g MgSO4 0.1 g MgSO4 0. 1 g KH2PO428.1000m CaCl 0.02 g Glucose 25 g pH7 Glucose 4-10 g Sodium acetate 20 g Trace elements(Fe, Co, Mn, Zn Amino acids(alanine, arginine, Cu, Ni, Mo) 2-10 ug each asparagine, aspartate, cysteine, Distilled water 1000 ml glutamate, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline. serine, threonine, tryptophan, tyrosine, valine) 100-200 ug of each Purines and pyrimidines (adenine, L guanine, uracil, xanthine) 10 mg of each itamins(biotin folate, nicotinic acid pyridoxal, pyridoxamine, pyridoxine, boflavin, thiamine, pantothenate p-aminobenoic acid) 0.01-1 mg of ea Trace elements(see first column) 2-10 ug each Distilled water 1000 ml pHZ a The photos are tubes of (a)the defined medium described, and(b) the complex medium described. Note how the complex medium is colored from the vari- ous organic extracts and digests that it contains