FCA is probably the most potent adjuvant but may be inappropriate for some purposes. Source: Reproduced from M.A. Kerr and R. Thorpe, Immunochemistry Labfax( 1994), Bios Scientific. Oxford Examples of immunization protocols that have been used successfully Species Priming First boostRest Subsequent eriod( weeks eriod(weeks Mice 5-100μg 2-3 50-100gg3 5-100pμg rats and antigen in antigen in antigen in Fia guinea FCA or FIA. other or PBS, i p, pigs other uvant or S.C.,l.m. or l\ adjuvant, PBS. im o s.C. or L. m. Rabbits|50-250μg|3-4 50-250ug 4 or longer 50-250pg antigen in antigen in antigen in FIa FCA or FIA or or PBS, i.m., other other S.C. or l\ adjuvant, uvant or i.d. i.m. or PBS, i. m, or s c Shee250μg-10mg4 250ug-10mg 4-8 or longer 250μg-10mg and goats antigen in antigen ir antigen in FIA FCA or FIA or or other other adjuvant, adjuvant. adjuvant, s.c. or I.v. s.c. or l.m. ors. c Horses 250ug-50mg 4 250Hg-50mg or longer250ug-50mg n Fl267 FCA is probably the most potent adjuvant but may be inappropriate for some purposes. Source: Reproduced from M.A.Kerr and R.Thorpe, Immunochemistry Labfax(1994), Bios Scientific, Oxford. Examples of immunization protocols that have been used successfully Species Priming Rest period(weeks) First boost Rest period(weeks) Subsequent boosts Mice, rats and guinea pigs 5-100g antigen in FCA or other adjuvant, s.c. or i.m. 2-3 50-100g antigen in FIA, other adjuvant or PBS, i.m. or s.c. 3 5-100g antigen in FIA or PBS, i.p., s.c., i.m. or i.v. Rabbits 50-250g antigen in FCA or other adjuvant, i.d., i.m. or s.c. 3-4 50-250g antigen in FIA or other adjuvant or PBS, i.m., or s.c. 4 or longer 50-250g antigen in FIA or PBS, i.m., s.c. or i.v. Sheep and goats 250g-10mg antigen in FCA or other adjuvant, i.m.,s.c. or i.d. 4 250g-10mg antigen in FIA or other adjuvant, i.m.or s.c. 4-8 or longer 250g-10mg antigen in FIA or other adjuvant, i.m., s.c.or i.v. Horses and 250g-50mg antigen in 4 250g-50mg antigen in 4-8 or longer 250g-50mg antigen in FIA