Ecc01092674 BstAPl 179 Aatll 2617 Ndel 183 Sspl 2501 Ehel 235 Pdml 2294 Bcgl 2215 2486 146/ 236/ lacZ MCS Scal 2177 469 pUC18/19 Sapl 683 2686bp 852 Aflll,BspLU111 806 Gs川1784 1626 G10I1779 1466 Ec031l1766 Ea110511694 Tep (pMB1) Cail 1217 9 9 pUC18/19