酶 识别位点 末端类型 末端序列 Alu I 5'-AGCT-3' 平端 5'-AG CT-3' 3'-TCGA-5' 3'-TC GA-5' Sau3A I 5'-GATC-3' 3'黏性末端 5'GATC-3' 3'-CTAG-5' 3'-CTAG-5' Hinf I 5'-GANTC-3' 3'黏性末端 5'-G ANTC-3' 3CTNAG-5' 3'-CTNA G-5' BamHI 5'-GGATCC-3' 3'黏性末端 5'-G GATCC-3' 3'-CCTAGG-5 3'-CCTAG G-5' BsrBI 5'-CCGCTC-3' 平末端 5'NNNCCGCTC3' 3-GGCGAG-5' 3-NNNGGCGAG-5' EcoR I 5'-GAATTC-3' 3'黏性末端 5'-G AATTC-3 3'-CTTAAG-5' 3'-CTTAA G-5' Not I 5-GCGGCCGC-3 3黏性末端 5'-GC GGCCGC-3' 3'-CGCCGGCG-5 3'-CGCCGG CG-5' BglI 5'-GCCNNNNNGGC-3' 5黏性末端 5'-GCCNNN NNGGC-3' 3'-CGGNNNNNCCG-5' 3'-CGGNN NNNCCG-5' N:为任一核苷酸。查阅REBASE(http://www.neb.com/rebase/rebase.html)可获得所有 限制性内切核酸酶的具体资料 图7-16限制性内切酶图 7 -16 限制性内切酶 9