Name Section Question 3 You find a plasmid that you think carries the harE gene, but you need to confirm that indeed the target gene has been inserted. When you made your library, you cut your genomic DNA with ecori and cloned it into a unique ecori restriction site in the vector a) How can you use the Ecori restriction enzyme to tell you if the gene has been inserted? the reco you find that the hare gene is in the plasmid, but now you want a restriction map of sample with EcoRI, the second sample with HindIll, and the third sample with both EcoRI and HindIll. Then you run the digested dNA on a gel to see the fragments ECORI ECoRI HindⅢ WindMIll 5.3 3.0 2.7 1.5 b)Considering that the hare gene is smaller than the vector, i)Circle the fragments on the gel that contain all or part of the hare gene ii)Draw the restriction map of this recombinant plasmid 7012Fall2003Name:___________________________________ Section:_____ 7.012 Fall 2003 4 Question 3 You find a plasmid that you think carries the harE gene, but you need to confirm that indeed the target gene has been inserted. When you made your library, you cut your genomic DNA with EcoRI and cloned it into a unique EcoRI restriction site in the vector. a) How can you use the EcoRI restriction enzyme to tell you if the gene has been inserted? Suppose you find that the harE gene is in the plasmid, but now you want a restriction map of the recombinant plasmid. You take three individual samples of the plasmid and digest one sample with EcoRI, the second sample with HindIII, and the thrird sample with both EcoRI and HindIII. Then you run the digested DNA on a gel to see the fragments. b) Considering that the harE gene is smaller than the vector, i) Circle the fragments on the gel that contain all or part of the harE gene. ii) Draw the restriction map of this recombinant plasmid