BEH.462/3. 962J Molecular Principles of Biomaterials Spring 2003 o Issues of maintaining cell viability and reproducibility in measurements o Issues of cell sources Often require primary cells in current systems Patterning cells for sensing 12 Techniques used o Photolithography o Microcontact printing(soft lithography) o Microfluidic patterning o Membrane lift-off (materiat ot pnM Stre desired panem Removal of stream I PDMS Str (Park and shuler, 2003) Dispense of Removal of Fsgs rm lcr ochametis s af the prar fow pamernnat. te ite n Cal pahterInthgraphy, (b) microcontact printing (e) microfluidic patterning soft lithography and self-assembled monolayers Techniques based on the formation of gold (or other metal)-thiol bonds and spontaneous assembly of close packed alkyl chain structures on a surface Lecture 20-Biosensors 5 of 8BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Lecture 20 – Biosensors 5 of 8 ƒ Cons o Issues of maintaining cell viability and reproducibility in measurements o Issues of cell sources ƒ Often require primary cells in current systems Patterning cells for sensing12 ƒ Techniques used: o Photolithography o Microcontact printing (soft lithography) o Microfluidic patterning o Membrane lift-off (Park and Shuler, 2003) soft lithography and self-assembled monolayers ƒ Techniques based on the formation of gold (or other metal)-thiol bonds and spontaneous assembly of close￾packed alkyl chain structures on a surface