激光共聚焦显微镜 Modern confocal microscopes can be considered as completely integrated electronic systems where the optical microscope plays a central role in a configuration that consists of one or more electronic detectors,a computer (for image display,processing,output,and storage),and several laser systems combined with wavelength selection devices and a beam scanning assembly. Photomultiplier Detector Confocal Microscopy Information Flow Schematic Diagram Detector Laser Scanning Pinhole Confocal Microscope Low Noise Frame Aperture Photomultiplier Optical Store Video Tube Out-of-Focus Display Video Fluorescence- Configuration Light Rays Scanning Prints. Computer Barrier Unit Slides Filter Excitation Laser Filter Excitation In-Focus Source Air Cooled Confocal Light Rays Krypton Pinhole Argon Laser Barrier Filter SYNC Dichromatic 3-D Reconstruction Mirror Dichroic Excitation Mirror Light Exciter Objective Rays Filter Light Source Galvanometer Pinhole X,Y Syncro Scanning Digital Hardcopy Aperture Unit Scan and Backup Control Stepper Motor Focal High NA IFocus X-Y Stage for Z-Series Planes Objective Figure 2 Lens Cover Slip Controller Shutter Optical System Control Confocal Pinhole Control Specimen Specimen- Slide ◆Laser Power Photomultiplier◆ Figure 5 Tube Gain Filter Wheels 9激光共聚焦显微镜 • Modern confocal microscopes can be considered as completely integrated electronic systems where the optical microscope plays a central role in a configuration that consists of one or more electronic detectors, a computer (for image display, processing, output, and storage), and several laser systems combined with wavelength selection devices and a beam scanning assembly. 9