荧光显微镜 The entire specimen is subjected to intense illumination from an incoherent mercury or xenon arc-discharge lamp,and the resulting image of secondary fluorescence emission can be viewed directly in the eyepieces or projected onto the surface of an electronic array detector or traditional film plane. Kohler lllumination in Reflected Light Fluorescence Image Field Plane Diaphragm Controls Field Collector Diameter Field Arc Lamp Lens Lens Plasma Ball -Emission (Barrier) Filter Cover Glass. Dichromatic Specimen- Mirror Microscope Slide Objective Widefield illumination Excitation Rear Focal (Large Volume) Beam Arc Image Excitation Plane Filter at Condenser Aperture Diaphragm Condenser and Objective Figure 5 10 Specimen-荧光显微镜 • The entire specimen is subjected to intense illumination from an incoherent mercury or xenon arc-discharge lamp, and the resulting image of secondary fluorescence emission can be viewed directly in the eyepieces or projected onto the surface of an electronic array detector or traditional film plane. 10