Yano et al Page 19 火大★ ★★★★ SPF 2 67「rT三 SPF+abx B. fragilis SFB ■GF+ASF 0.5 0.0 GF+Bd SPF GFGF BF GF sp. Bd n s ns ★★★“★★★ *p=0.0517 1.0 0.5 SPF SPF GFGFGF SPFSPF GFGFGF Sp +s PCPA: PCPA:-+ Figure 3. Indigenous Spore-forming Bacteria Induce Colon 5-HT Biosynthesis and Systemic 5- HT Bioavailability (A) Levels of serum 5-HT. Data are normalized to serum 5-HT levels in SPF mice. SPF n=13: GF. n=17: GF+conv =P21 conventionalization n=4: SPF+Abx= P42 antibiotic treatment, n=7; B fragilis monoassociation(BF), n=6: SFB=Segmented Filamentous Bacteria monoassociation, n=4; ASF=Altered Schaedler Flora P21 colonization, n=4 Sp=spore-forming bacteria, P21 colonization, n=4; B uniformis P21 colonization, n=4, Bacteroides consortium. n=3 )Levels of colon 5-HT relative to total protein. Data are normalized to colon 5-H relative to total protein in SPF mice. n=5-15 (C) Levels of colon 5-HT relative to total protein after intrarectal treatment with the Tph inhibitor. PCPA or vehicle. n=4 Cell. Author manuscript; available in PMC 2016 April 09Figure 3. Indigenous Spore-forming Bacteria Induce Colon 5-HT Biosynthesis and Systemic 5- HT Bioavailability (A) Levels of serum 5-HT. Data are normalized to serum 5-HT levels in SPF mice. SPF, n=13; GF, n=17; GF+conv.=P21 conventionalization, n=4; SPF+Abx= P42 antibiotic treatment, n=7; B. fragilis monoassociation (BF), n=6; SFB=Segmented Filamentous Bacteria monoassociation, n=4; ASF=Altered Schaedler Flora P21 colonization, n=4; Sp=spore-forming bacteria, P21 colonization, n=4; B. uniformis P21 colonization, n=4; Bd=Bacteroides consortium, n=3. (B) Levels of colon 5-HT relative to total protein. Data are normalized to colon 5-HT relative to total protein in SPF mice. n=5–15. (C) Levels of colon 5-HT relative to total protein after intrarectal treatment with the Tph inhibitor, PCPA, or vehicle. n=4. Yano et al. Page 19 Cell. Author manuscript; available in PMC 2016 April 09. Author Manuscript Author Manuscript Author Manuscript Author Manuscript