Technique Use transposon vector containing lac fusion Carries promoterless lacz gene and selectable antibiotic marker Selection for antibiotic marker generates set of random insertions in chromosome Colonies carrying transposon screened for expression under conditions tested BIOL 533 Lecture 4BIOL 533 4 Lecture 4 Technique • Use transposon vector containing lac fusion – Carries promoterless lacZ gene and selectable antibiotic marker – Selection for antibiotic marker generates set of random insertions in chromosome – Colonies carrying transposon screened for expression under conditions tested