Reporter gene fusions Gene fusion translational Promoter and translational signal from foreign gene Initiation signal/+NH, coding sequence Translational signal also from reporter gene Initiation sequence and part of NH sequence of reporter gene missing but COOH region still present Two Coding sequences must be in frame BIOL 533 Lecture 4BIOL 533 3 Lecture 4 Reporter Gene Fusions • Gene fusion: translational – Promoter and translational signal from foreign gene • Initiation signal/+NH2 coding sequence – Translational signal also from reporter gene • Initiation sequence and part of NH2 sequence of reporter gene missing, but COOH region still present – Two coding sequences must be in frame