1056 ##100#6521.0 95100#000.0 050010865.0 PI600#021L.0 #S200#0111.0 9500车5960 25600-00005.0 #LS00+062.0 6S000斤0660.0 O1500#895c0 8010.0405900 1 EVD+HS 4299.0+755h 09260+880 616.1#z06s 21201f0ss材 2910:0#086t EIL'I F ELSE C.#609 VD+HS (-13)1431Md 22990#105g 6151#206s 2120Tt0859 BILb'I F ELSE 8#260g 0760:0#2860:0 P00.0128090 3620.:0#0698.0 1:010869.0 2e30.0#8169.0 2960.0-0600:0 28100+1Lc2.0 P话00.0H-000.0 P600:0+006.0 2600.0+00500 010.0-000.0 g600.012280.0 1-90.00000.0 e100F6100 (1=13)1431M Kd 9119.5#060:490.0午0060.0 2963.1-#8559 男 29171-#15s p99.0+001k1 RC材0：010005：0 #t2S+Lh6t#材600+0020.1 P9K6-#519.00材0040010.1 hS/#09:26900#70000 580:1-4S0#9:24500限m #650:0+0009.0 (1-13)1431M d 299.10006 4775052590 #290:0#51Sr0 #1100F0LS-r0 7900.0斤00000.0 24材0：010000.5 S5005105.0 19900#05s-0 (uuoxysAB :SOV)suasauDo wmadsounT Jo soo eu u (M-3 3w)sE'SOY Jo uuo a:onuoD o1 3upoooe 'uaj!p Apueoy!uays iou aom n wes a q pamolloj uunjoo aus e urm sanpe 'uouean ad saeoydal omi ym siuawuadxo aom Jo S sueau are sanpeA S1195#00s 23101#Et5s t21#7#6t 2SS0.0-0060.0 PA50:01000:0 #e100FE6h1:0 AA50:040000.0 3K51#509.028910400160 S0.2c00+0.0594L.13+P a0-1-#S真。1500#7961.0 38+93 q9EE'IF090 R775052590 PS8Z'F EZ8'S #929.0#7060 240050000 4600.010080.0 2200:0+06500 20200-80000 F210.0+06900 2:250#089.s #620-F50.c 2055:0#788.h 2600:0#720h #1K-#090s 9I'I00'S 2S59060505 26901--501s 2051017085 凸 8600:0170.h qILE'I080'S 295-T#000c (uuoysnqos!:SgI)suasauo wmuiadsoun7 Jo sioo u (M 3w)8I SgI Jo luoa:JonuoD U 工 工1056 Table 2 Hairy roots of Lithospermum canescens clone Lc1 dry weight and content of acetylshikonin (mg g−1 DW) estimated after 6 weeks of culture Medium LS LS + GA3 SH SH + GA3 Line of hairy roots Dry weight (g l−1) ACS Dry weight (g l−1) ACS Dry weight (g l−1) ACS Dry weight (g l−1) ACS A 5.650 ± 0.923c 0.1513 ± 0.062a 4.060 ± 1.336b 0.6200 ± 0.062b 5.640 ± 2.611b 0.7482 ± 0.097e 4.957 ± 0.862b 0.1233 ± 0.014a B 5.103 ± 1.069a 0.1870 ± 0.011a 4.547 ± 1.223c 1.4100 ± 0.88e 6.573 ± 1.898c 0.4632 ± 0.064d 6.183 ± 0.923c 0.1760 ± 0.013b C 4.887 ± 0.770a 0.4000 ± 0.078b 5.110 ± 1.355c 0.4700 ± 0.042a 5.723 ± 1.018b 0.8390 ± 0.024f 5.907 ± 1.919c 0.3260 ± 0.087c D 4.327 ± 0.709b 2.7200 ± 0.048c 3.652 ± 0.522b 1.0260 ± 0.094d 4.947 ± 1.523a 0.8480 ± 0.112f 4.550 ± 1.027b 0.7120 ± 0.091d E 5.080 ± 1.371a 0.1554 ± 0.025a 4.677 ± 1.350d 1.0370 ± 0.247d 7.673 ± 1.976d 0.1267 ± 0.067a 4.980 ± 2.578b 0.1110 ± 0.025a F 5.597 ± 2.348c 0.1552 ± 0.036a 5.823 ± 2.285e 0.6667 ± 0.089b 7.183 ± 2.154d 0.3913 ± 0.083c 5.785 ± 1.488c 0.1265 ± 0.046a G 4.295 ± 1.247b – 3.207 ± 0.628a 0.7763 ± 0.164c 5.143 ± 1.106a 0.7720 ± 0.093e 3.573 ± 1.471a 0.3600 ± 0.093c H 5.040 ± 1.146a – 5.580 ± 2.014e 0.3000 ± 0.039a 6.547 ± 1.471c 0.2271 ± 0.073b 6.097 ± 1.327c 0.1290 ± 0.087a Control: the content of ACS 24.35 (mg g−1 DW) in natural roots of Lithospermum canescens (ACS: acetylshikonin) Values are means ± SE of three experiments with two replicates per treatment. Values within a single column followed by the same letter were not significantly different, according to the LSD at the 5% level Table 3 Hairy roots of Lithospermum canescens clone Lc1 dry weight and content of isobutyrylshikonin (mg g−1 DW) estimated after 6 weeks of culture Medium LS LS + GA3 SH SH + GA3 Line of hairy roots Dry weight (g l−1) IBS Dry weight (g l−1) IBS Dry weight (g l−1) IBS Dry weight (g l−1) IBS A 5.650 ± 0.923c 0.0737 ± 0.053c 4.060 ± 1.336b 0.2900 ± 0.033c 5.640 ± 2.611b 0.1200 ± 0.005d 4.957 ± 0.862b Trace B 5.103 ± 1.069b 0.0800 ± 0.009c 4.547 ± 1.223c 0.4067 ± 0.046d 6.573 ± 1.898c 0.1200 ± 0.009d 6.183 ± 0.923c – C 4.887 ± 0.770b 0.0540 ± 0.072b 5.110 ± 1.355c 0.1493 ± 0.013a 5.723 ± 1.018b 0.0500 ± 0.002c 5.907 ± 1.919c 0.0296 ± 0.003a D 4.327 ± 0.709a 0.0773 ± 0.027c 3.652 ± 0.522b 0.3067 ± 0.048c 4.947 ± 1.523a 0.1000 ± 0.011e 4.550 ± 1.027b 0.1333 ± 0.031c E 5.080 ± 1.371b 0.0392 ± 0.012a 4.677 ± 1.350c 0.4107 ± 0.063d 7.673 ± 1.976d 0.0322 ± 0.009b 4.980 ± 2.578b – F 5.597 ± 2.348c – 5.823 ± 2.285d 0.2200 ± 0.033b 7.183 ± 2.154d 0.1250 ± 0.014d 5.785 ± 1.488c Trace G 4.295 ± 1.247a – 3.207 ± 0.628a 0.1967 ± 0.031b 5.143 ± 1.106a 0.2000 ± 0.035f 3.573 ± 1.471a 0.0625 ± 0.016b H 5.040 ± 1.146b – 5.580 ± 2.014d 0.1433 ± 0.023a 6.547 ± 1.471c 0.0179 ± 0.013a 6.097 ± 1.327c – Control: the content of IBS 14.48 (mg g−1 DW) in natural roots of Lithospermum canescens (IBS: isobutyrylshikonin) Values are means ± SE of three experiments with two replicates per treatment. Values within a single column followed by the same letter were not significantly different according to the LSD at the 5% level