Hydrolysis of Complex Carbohydrates General mechanism of polysaccharide hydrolysis by glycosidases (a)Initial formation of an oxonium ion is followed by either one inversion or two (a)Initial oxonium ion formation CH2OH CH2OH OH CH2OH OH Bottom face shielded Oxonium ion by leaving group HO CH2OH OH HO 0之 HO Enz Cengage Learning.All Rights Reserved. General mechanism of polysaccharide hydrolysis by glycosidases (a) Initial formation of an oxonium ion is followed by either one inversion or two Hydrolysis of Complex Carbohydrates