Essentiality of reactions in metabolic core Two types of reactions in metabolic core Reactions that are essential for growth under all conditions H. pylori: no data in the paper E. coli: 81 out of 90 n Experimental data: 747o of the enzymes that catalyze core metabolic reactions are essential, compared with a 19.6%o lethality fraction of the noncore enzymes S cerevisiae: all 33 Experimental data: 84%o of the core enzymes are essential, whereas 15.6%o of noncore enzymes are essential Reactions that are required for optimal metabolic performance a When assuming a 10%o reduction in the growth rate the size of the metabolic core becomes 83 in E. coliEssentiality of reactions in metabolic core ◼ Two types of reactions in metabolic core ◼ Reactions that are essential for growth under all conditions ◼ H. pylori: no data in the paper ◼ E. coli: 81 out of 90 ◼ Experimental data: 74.7% of the enzymes that catalyze core metabolic reactions are essential, compared with a 19.6% lethality fraction of the noncore enzymes. ◼ S. cerevisiae: all 33 ◼ Experimental data: 84% of the core enzymes are essential, whereas 15.6% of noncore enzymes are essential. ◼ Reactions that are required for optimal metabolic performance ◼ When assuming a 10% reduction in the growth rate, the size of the metabolic core becomes 83 in E. coli