第三节：转录组数据核心分析 00M队 质量控制 去接头；过滤 低质量reads FastQC-测序质量评估 FASTX-Toolkit,Trimmomatic--一质量控制 eFastQC Report Summary Per base sequence quality Basic Statistics Quality scores across all bases (Sanger /lllumina 1.9 encoding) 40 Per base sequence quality 3 Per tile sequence quality Per sequence quality scores 34 Per base sequence content Per sequence GC content ⑦Per base N content Sequence Length Distribution 66420 Sequence Duplication Levels Overrepresented sequences ①Adapter Content ⑧Kmer Content 64120 8 6 4 2 0 12345678912-1318-1924-2530-3136-3742-4348-4954-55606166-6772-7378-79B4-85909196-97 Position in read(bp》 11第三节：转录组数据核心分析 11 FastQC—测序质量评估 FASTX-Toolkit，Trimmomatic—质量控制 质量控制 去接头；过滤 低质量reads