第三节：转录组数据核心分析 00 、数据获取 GSA THE CANCER GENOME ATLAS NIH National Cancer Institute National Human Genome Research Institute Genome Sequence Archive NCBI SRA TCGA/GDC(cancer) ArrayExpress NIH NATIONAL CANCER INSTITUTE Genomic Data Commons fastq-dump EBI ArrayExpress (SRAToolkit) 公共数据库 测序公司 Fastq文件格式： esRR3418005,1HAL:1262:D2 EWTACXX:8:1101:1602:21361 ength=100 GGCAAGATCTGATCTCTCAGCAACTCAATTACAACCATAACCGCGTGTGACTTCTAAGCC +SRR3418005,1HAL:1282:D2 EWTACXX:8:1101:1602:21361 ength-100 ::1DDAD?DDFHFGHII EIIIGHHHDHGIGDFGHGGEGIIHGBEDHEEGGFIDEEAAEH Q8RR3418005.2HAL:1282:D2 EWTACXX:8:1101:1550:21931 ength=100 ATCTGATTCAATCATAAATTTTACACAATCAATTTGTCGGTACTCTCCTTTTGGTCATAT +sRR3418005,2HAL:12B2:D2 EWTACXX:8:1101:1550:21931 ength=100 <?0BDD:DCDDHHGIBGA<FFF<:FDGGCE9CADHIEG:?DF):BBB9??BGG60?B<B- @sRR3418005,3HAL:1282:D2 EWTACXX:8:1101:1632:22051 ength-100 AGACGCTCGTACCAAATCCGTTACCGTCTCCGTCGTTACCTCCTCCTTCGCGACGGGAAC +sRR3418005.3HAL:12B2:D2 EWTACXX:8:1101:1632:22051 ength=100 --+:A@DDE@F8C<A+CBEFIIFIF@DFFFFFDF(@F--.-70FEF).-@A## esRR3418005.4HAL:1282:D2 EWTACXX:8:1101:1588:22271 ength=100 CTCATTTTTATTACCGCATATATGACATATGATCAATTACATAAAGAAGCAAATCTTAGO +SRR3418005,4HAL:12B2:D2 EWTACXX:8:1101:158B:22271 ength-100 30-DDDAFHFAHEH?FF<EBF9EHDHHGOACCGICHHCHGIEHG<DFB9DDFHEGFHDG 0sRR3418005.5HAL:1282:D2 EWTACXX:8:1101:1991:21131 ength=100 CGGAAGCAGCTGAGAAGCCTCATGGTTACCAACAAGAGCATCCTCATCAGTTNCACCATA +9RR3418005.5HAL:1282:D2 EWTACXX:8:1101:1991:21131 ength=100 GCOFFFDFHHHFGIIGIGIJJJJIJIIIIIIJJJJCEIGIIJHIJEIIJJHI#-<FFHIJ 10第三节：转录组数据核心分析 10 Fastq文件格式： NCBI SRA EBI ArrayExpress TCGA/GDC(cancer) fastq-dump (SRAToolkit) 公共数据库 测序公司 数据获取