3.Perform coagulase test on S.aureus and S.epidermidis.Add 0.5 ml of diluted rabbit plasma to small sterile tube.Add 0.5 ml of S.aureus or S.epidermidis to the tube of plasma.Incubate in the 37C waterbath,and examine at the end of the lab period. 4.Perform the catalase test on S.aureus and E.faecalis.Take a loop full of colonies from a plate of each bacterium and place inoculum separately on a glass microscope slide.Add a drop of 3%hydrogen peroxide to each. Immediate vigorous oxygen bubbles indicates catalase-positive bacteria. 56 3. Perform coagulase test on S. aureus and S. epidermidis. Add 0.5 ml of diluted rabbit plasma to small sterile tube. Add 0.5 ml of S. aureus or S. epidermidis to the tube of plasma. Incubate in the 37o C waterbath, and examine at the end of the lab period. 4. Perform the catalase test on S. aureus and E. faecalis. Take a loop full of colonies from a plate of each bacterium and place inoculum separately on a glass microscope slide. Add a drop of 3% hydrogen peroxide to each. Immediate vigorous oxygen bubbles indicates catalase-positive bacteria