FIRST PERIOD Material: 1.One mannitol salt agar (MSA)plate 2.One blood agar plate 3.Overnight broth cultures of Staphylococcus aureus,Staphylococcus epidermidis,and Enterococcus faecalis (on plate) 4.Sterile swabs and sterile toothpicks 5.Rabbit plasma 6.1.0 and 0.1 ml pipets 7.3%Hydrogen peroxide solution Procedure:(work in pairs) 1.Swab an area of the face (e.g.,cheek or forehead)and streak a mannitol salt agar plate.Incubate the plate at 37C for 48 hours. 2.Use a sterile toothpick to scrape tooth enamel.Using the toothpick,make a single short streak on a blood agar plate.Then,using your inoculating loop, streak out the toothpick streaks as illustrated below.Alternatively,you can use a sterile swap to do a back of the throat swap for strep-throat.Inoculate Enterococcus faecalis to one quadrant on the blood agar plate as a negative control for hemolysis.Incubate the plates in anaerobic jar at 37C for 48 hours 5555 FIRST PERIOD Material: 1. One mannitol salt agar (MSA) plate 2. One blood agar plate 3. Overnight broth cultures of Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis (on plate). 4. Sterile swabs and sterile toothpicks 5. Rabbit plasma 6. 1.0 and 0.1 ml pipets 7. 3% Hydrogen peroxide solution Procedure: (work in pairs) 1. Swab an area of the face (e.g., cheek or forehead) and streak a mannitol salt agar plate. Incubate the plate at 37°C for 48 hours. 2. Use a sterile toothpick to scrape tooth enamel. Using the toothpick, make a single short streak on a blood agar plate. Then, using your inoculating loop, streak out the toothpick streaks as illustrated below. Alternatively, you can use a sterile swap to do a back of the throat swap for strep-throat. Inoculate Enterococcus faecalis to one quadrant on the blood agar plate as a negative control for hemolysis. Incubate the plates in anaerobic jar at 37°C for 48 hours