and South East Asia22230 sinensis contains several bioactive components, and many reports have carefully analyzed its make-up of polysaccharides, sterols, nucleosides and protein 2224. Many of these components have been found to possess immunological, anti-tumorigenic, anti-oxidative, anti-inflammatory, anti-fatigue anti-fungal and anti-hypertensive properties, as well as to protect the kidney, liver and lung2526. However, there is no systematic analysis of the drug-target network of O sinensis. Therefore, the aim of the current study was to explain how the immunoregulation function was implemented and find a component that can act as an adjuvant of the hepatitis b vaccine through systems pharmacology analysis. Briefly, as seen in Figure 1, we first use a network pharmacology approach to determine the active ingredients of O. sinensis. A molecular-target network was then developed followed by enrichment analysis and functional classification. After screening,a single component was selected and validated by in vivo and in vitro experiments Results Candidate component identification. It has been difficult to identify the mechanisms of action of different TCM agents due to their complex biochemical make-up, and specific methods to identify the bioactive compounds have not been available until now. In this study, we used oral bioavailability(OB) screening and drug-likeness(DL) property evaluation or drug half-life(HL) prediction to identify the bioactive compounds".All the ingredients of O. sinensis were obtained from the Traditional Chinese Medicines Systems Pharmacology Database and Analysis4 and South East Asia22,23. O. sinensis contains several bioactive components, and many reports have carefully analyzed its make-up of polysaccharides, sterols, nucleosides and protein21,22,24. Many of these components have been found to possess immunological, anti-tumorigenic, anti-oxidative, anti-inflammatory, anti-fatigue, anti-fungal and anti-hypertensive properties, as well as to protect the kidney, liver and lung25,26. However, there is no systematic analysis of the drug-target network of O. sinensis. Therefore, the aim of the current study was to explain how the immunoregulation function was implemented and find a component that can act as an adjuvant of the hepatitis b vaccine through systems pharmacology analysis. Briefly, as seen in Figure 1, we first use a network pharmacology approach to determine the active ingredients of O. sinensis. A molecular-target network was then developed, followed by enrichment analysis and functional classification. After screening, a single component was selected and validated by in vivo and in vitro experiments. Results Candidate component identification. It has been difficult to identify the mechanisms of action of different TCM agents due to their complex biochemical make-up, and specific methods to identify the bioactive compounds have not been available until now27. In this study, we used oral bioavailability (OB) screening and drug-likeness (DL) property evaluation or drug half-life (HL) prediction to identify the bioactive compounds7,9. All the ingredients of O. sinensis were obtained from the Traditional Chinese Medicines Systems Pharmacology Database and Analysis