课程代码:BIOL11003504课程名称:改变生活的生物技术姓名:罗啸学号:15307130248 miehei脂肪酶选择性催化EPA的转酯反应,使EPA以EPA乙酯的形式富集,而DHA存 在于鱼油的甘油酯上,经过下步的短程蒸馏分离,可以使DHA和EPA分离,EPA乙酯浓 缩物经色谱法纯化可以得到纯品;而富集DHA的甘油酯在 Candida antarctica脂肪酶催 化下与乙醇发生转酯反应,可得到DHA乙酯浓缩物,再经色谱纯化可得到DHA纯品。 5参考文献 [1] Kinsella, J E, Food components with potential therapeutic benefits: the n-3 polyunsaturated fatty acids of fish oils[J] Food Technology, 1986, 40: 89 2] Simopoulos AP, Omega-3 fatty acids in health and disease and in growth and development Amer J Clin Nutr. 1991.54: 438 3] Yang LY, Kuksis A. Myher JJ. Lumenal hydrolysis of menhaden and rapeseed oils and their fatty acid methyl and ethyl esters in the rat[J]. Biochem Cell Biol. 1989 Apr-May67(4-5)192-204 14 Larry D. Lawson, Bronwyn G. Hughes. Human absorption of fish oil fatty acids as triacylglycerols, free acids, or ethyl esters[J]. Biochem Biophys Res Comm, 1988.152: 328s [5]吴可克.酶促鱼油选择性水解制备EPA、DHA甘油酯的研究J].中国油脂.200203) []李金章,王玉明,薛长湖,孙兆敏,楼乔明.脂肪酶催化乙酯甘油酯酯交换制备富含EPA 和DHA的甘油三酯中国油脂.201101) [刁]唐青涛氽若黔宗敏华黄祥斌,王艳菊.脂肪酶富集DHA和EPA的研究进展门,华西药 学杂志,2001(04) 8] Shimada Y, Sugihara A, Nakano H,et al. Purification of docosahexaenoicacid by selective esterification of fatty acids from tuna oil with Rhizopusdelemar lipase[J]. J Am Oil Chem Soc,1997,74:97 9] Cerdan LE, Medina AR, Gimenez AG, et al. Synthesis of polyunsaturatedfatty acid-enriched triglycerides by lipase-catalyzed esterification[ J]. J Am Oil Chem Soc, 1998, 75: 1329 [10] Wanasundara U, Shahidi F Lipase-assisted concentration of n-3 polyun-saturated fatty acid in acylglycerols from marine oils[ J]. J Am Oil Chem Soc, 1998, 75: 945 [11] Breivik H, Haraldsson GG, Kristinsson B PrEPAration of highly purifiedconcentrates of eicosapentaenoic acid and docosahexaenoic acid J AmOil Chem Soc, 1997, 74: 1425 Breivik H, Haraldsson GG, Kristinsson B PrEPAration of highly purifiedconcentrates of eicosapentaenoic acid and docosahexaenoic acid[]J AOil Che Soc, 1997,74: 1425课程代码：BIOL110035.04 课程名称：改变生活的生物技术 姓名：罗啸 学号：15307130248 miehei 脂肪酶选择性催化 EPA 的转酯反应,使 EPA 以 EPA 乙酯的形式富集,而 DHA 存 在于鱼油的甘油酯上,经过下步的短程蒸馏分离,可以使 DHA 和 EPA 分离,EPA 乙酯浓 缩物经色谱法纯化可以得到纯品;而富集 DHA 的甘油酯在 Candida antarctica 脂肪酶催 化下与乙醇发生转酯反应,可得到 DHA 乙酯浓缩物,再经色谱纯化可得到 DHA 纯品 11。 5 参考文献 [1] Kinsella, J.E, Food components with potential therapeutic benefits: the n-3 polyunsaturated fatty acids of fish oils[J] Food Technology, 1986,40:89 [2] Simopoulos AP, Omega-3 fatty acids in health and disease and in growth and development[J]. Amer J Clin Nutr, 1991,54:438 [3] Yang LY, Kuksis A. Myher JJ. Lumenal hydrolysis of menhaden and rapeseed oils and their fatty acid methyl and ethyl esters in the rat[J]. Biochem Cell Biol. 1989 Apr-May;67(4-5):192-204. [4] Larry D. Lawson, Bronwyn G. Hughes. Human absorption of fish oil fatty acids as triacylglycerols, free acids, or ethyl esters[J]. Biochem Biophys Res Comm, 1988.152:328s [5] 吴可克. 酶促鱼油选择性水解制备 EPA、DHA 甘油酯的研究[J]. 中国油脂. 2002(03) [6] 李金章,王玉明,薛长湖,孙兆敏,楼乔明. 脂肪酶催化乙酯甘油酯酯交换制备富含 EPA 和 DHA 的甘油三酯[J]. 中国油脂. 2011(01) [7] 唐青涛,余若黔,宗敏华,黄祥斌,王艳菊. 脂肪酶富集 DHA 和 EPA 的研究进展[J], 华西药 学杂志, 2001 (04). [8] Shimada Y,Sugihara A,Nakano H,et al.Purification of docosahexaenoicacid by selective esterification of fatty acids from tuna oil with Rhizopusdelemar lipase[J].J Am Oil Chem Soc,1997,74:97 [9] Cerdan LE,Medina AR,Gimenez AG,et al.Synthesis of polyunsaturatedfatty acid-enriched triglycerides by lipase-catalyzed esterification[ J].J Am Oil Chem Soc,1998,75:1329 [10] Wanasundara U,Shahidi F.Lipase-assisted concentration of n-3 polyun-saturated fatty acid in acylglycerols from marine oils[ J].J Am Oil Chem Soc,1998,75:945 [11] Breivik H,Haraldsson GG,Kristinsson B.PrEPAration of highly purifiedconcentrates of eicosapentaenoic acid and docosahexaenoic acid[J].J AmOil Chem Soc,1997,74: 1425 11 Breivik H,Haraldsson GG,Kristinsson B.PrEPAration of highly purifiedconcentrates of eicosapentaenoic acid and docosahexaenoic acid[J].J AmOil Chem Soc,1997,74: 1425