1期 程红焱等：超干种子耐贮藏性的细胞学及生理生化基础 17 度不同，如何建立数学模型确定种子的最佳贮藏条件以及准确预测种子的贮藏寿命以便及 时更新保存样本；(2)在超干贮藏过程中如何有效地控制自由基活性氧的毒害反应；(3) 促进种子细胞玻璃化的因子是什么；以及(4)超干种子在随后重新吸胀过程中的修复机 制：这些问题有可能成为今后超干种子贮藏研究的新方向。 惨考文献) Apel K.Hirt H.2004.Reactive oxygen ecies:metabolism,oxidative stress,and signal transduction[Rer Plant Bio,55:373-399 Bewley JD.1997.Seed germination and dormancy Pam Cell:105506 Buitink J.Claessens MMAE.Hemminga MA.et al.1998a.Influence of water content and temperature on molecular mobil ity and intracellular glasses in sceds and pollens [J].Plant Physiol,118:531-541 BuitinkJ Walters C.Hoekstra FA.a 1998b.Storage behavior of Typha latifolia pollen at low water contents:inter ity and gl cepts Pkysiol Plant,103:145-53 predict lifespan of dry germplasm [J.Plant Physiol,97:2385-2390 Buitink J.Leprince O.Hoekstra FA,et al,2000b.Dehydratiorrinduced redistribution of amphiphilic molecules between cy toplasm and lipids is associated with desiccation tolerance in seeds [J].Plant Plnsiol.124:1413-426 Chang BS,Beauvais RM,Dong A..Physical factors affec ting the s stability of fro -dried interleuki nd Biophys ,331:249258 Cheng HY(程红族).Song SQ(宋松泉).2004.Research perspect n bo-thermodyna of seed and pollen cell mem brane changes induced by desiccation [J1.JP列atP%siol Mol Biol(植物生理与分子生物学学报)，30(2)：127 135 Cheng HY ()1994.Studies on germplasm of seed utradrving storage.Ph.D thesis,Institute of Botany I M] Cheng HY(程红族).heng GH(郑光华).QmH(秦)红tal.196 nd its ultradry storage effects [J].Sei Agric Sin(中固农业科学)，29：653 Cheng HY(程红焱)，Zheng GH(郑光华)，TmN(嘉龄)，tal,I99l.Physiological,biochemical and utrastru tural sudieson utradriedseeds of.AaaP%toplysiol Sin(植物生理学报)，17：273一284 Cheng HY(程红，2 heng GH(郑光华)，TmN(陶毫龄)，eal,990.,cell seedsP.Pan es Newsletter.83/84:9 Cheng HY(程红焱)，Zheng GH(郑光华)，TamN(陶嘉龄)，al,1997.Storage of peanu seeds with w misture content for 11 vears in ambient temperature [J.Plant Genetic Resources Newsletter.110:35-40 Crowe JH,Hoekstra FA,Crowe LM,1992.Anhydrobiosis []4nn Rev Plant Physiol,54:570-599 Crowe LM.Reid DS..Is pres ving dry biomaterials []BiophysJ,71:2087- 2003 HeyesJA.Sealey DF.Vre de LA,1997.Plasma membrane ATPase activity during pepino ()ripening 目】.Plysiol Plant,101:570576 Hoekstra FA,Wolkers WF,BuitinkJ,etal,1997.Membrane stabilization in the dried state []Comp Bicchem Plrys 以1174·335341 hJS(胡家恕).ZhuC(朱诚).99.Tect of.4 al5im(植物生理学报)，25： 17117 Leprince.Harren FM..Metablic dysfunction and unabated respiration precede the ssof mem brane integrity during dehydration of germinating radicles [J.Plant Plsiol,122:597-608 1995-2005 Tsinghua Tongfang Optical Disc Co Ld.All rights reserved度不同 , 如何建立数学模型确定种子的最佳贮藏条件以及准确预测种子的贮藏寿命以便及 时更新保存样本 ; (2) 在超干贮藏过程中如何有效地控制自由基活性氧的毒害反应 ; (3) 促进种子细胞玻璃化的因子是什么 ; 以及 (4) 超干种子在随后重新吸胀过程中的修复机 制 ; 这些问题有可能成为今后超干种子贮藏研究的新方向。 〔参 考 文 献〕 Apel K , Hirt H , 2004. Reactive oxygen species : metabolism , oxidative stress , and signal transduction [J ] . A nnu Rev Plant Bio , 55 : 373 —399 Bewley JD , 1997. Seed germination and dormancy [J ] . Plant Cell , 9 : 1055 —1066 Buitink J , Claessens MMAE , Hemminga MA , et al , 1998a. Influence of water content and temperature on molecular mobil2 ity and intracellular glasses in seeds and pollens [J ] . Plant Physiol , 118 : 531 —541 Buitink J , Walters C , Hoekstra FA , et al , 1998b. Storage behavior of Typha latif olia pollen at low water contents : inter2 pretation on the basis of water activity and glass concepts [J ] . Physiol Plant , 103 : 145 —153 Buitink J , Leprince O , Hemminga MA , et al , 2000a. Molecular mobility in the cytoplasm : An approach to describe and predict lifespan of dry germplasm [J ] . Plant Physiol , 97 : 2385 —2390 Buitink J , Leprince O , Hoekstra FA , et al , 2000b. Dehydration2induced redistribution of amphiphilic molecules between cy2 toplasm and lipids is associated with desiccation tolerance in seeds [J ] . Plant Physiol , 124 : 1413 —1426 Chang BS , Beauvais RM , Dong A , et al , 1996. Physical factors affecting the storage stability of freeze2dried interleukin21 receptor antagonist : glass transition and protein conformation [J ] . A rch Biochem Biophys , 331 : 249 —258 Cheng HY (程红焱) , Song SQ (宋松泉) , 2004. Research perspective in bio2thermodynamics of seed and pollen cell mem2 brane changes induced by desiccation [J ] . J Plant Physiol Mol Biol (植物生理与分子生物学学报) , 30 (2) : 127 — 135 Cheng HY (程红焱) , 1994. Studies on germplasm of seed ultradrying storage. Ph. D thesis , Institute of Botany [ M ] . Beijing : Chinese Academy of Sciences Cheng HY (程红焱) , Zheng GH (郑光华) , Qin H (秦红) , et al , 1996. Water thermodynamic analysis on seeed desic2 cation toleranceand its ultradry storage effects [J ] . Sci A gric Si n (中国农业科学) , 29 : 65 —73 Cheng HY (程红焱) , Zheng GH (郑光华) , Tao KL (陶嘉龄) , et al , 1991. Physiological , biochemical and ultrastruc2 tural studies on ultradriedseeds of some B rassica species [J ] . Acta Phytophysiol Si n (植物生理学报) , 17 : 273 —284 Cheng HY (程红焱) , Zheng GH (郑光华) , Tao KL (陶嘉龄) , et al , 1990. Effects of ultradrying on ageing , cell ultra2 structure and vigor of Chinese cabbage seeds [J ] . Plant Genetic Resources Newsletter , 83/ 84 : 9 —14 Cheng HY (程红焱) , Zheng GH (郑光华) , Tao KL (陶嘉龄) , et al , 1997. Storage of peanut seeds with low moisture content for 11 years in ambient temperature [J ] . Plant Genetic Resources Newsletter , 110 : 35 —40 Crowe J H , Hoekstra FA , Crowe LM , 1992. Anhydrobiosis [J ] . A nn Rev Plant Physiol , 54 : 570 —599 Crowe LM , Reid DS , Crowe J H , 1996. Is trehalose special for preserving dry biomaterials [J ] ? Biophys J , 71 : 2087 — 2093 Heyes JA , Sealey DF , Vre de LA , 1997. Plasma membrane ATPase activity during pepino ( S planum m uricat un) ripening [J ] . Physiol Plant , 101 : 570 —576 Hoekstra FA , Wolkers WF , Buitink J , et al , 1997. Membrane stabilization in the dried state [J ] . Comp Biochem Physi2 ol , 117A: 335 —341 Hu J S (胡家恕) , Zhu C (朱诚) , 1999. Effect of aging2resistance of ultradried safflower seeds and its mechanism [J ] . Ac2 ta Phytophysiol Si n (植物生理学报) , 25 : 171 —177 Leprince O , Harren FJ M , Buitink J , et al , 2000. Metabolic dysfunction and unabated respiration precede the loss of mem2 brane integrity during dehydration of germinating radicles [J ] . Plant Physiol , 122 : 597 —608 1 期 程红焱等 : 超干种子耐贮藏性的细胞学及生理生化基础 71 © 1995-2005 Tsinghua Tongfang Optical Disc Co., Ltd. All rights reserved