Plasmid Conjugative: plasmids which govern their own transfer by cell-to-cell contact are called conjugative Tra region: a set of genes within the plasmid that control the transmissability by conjugation Hfr(high frequency of recombination): strains of bacteria that transfer large amounts of chromosomal dna during conjugation Supercoil: plasmids isolated from the cells are in supercoiled configuration Curing of plasmids: elimination of plasmids from host cel Plasmid separation: by ultracentrifugation or electrophores by various treatmentsPlasmid • Conjugative: plasmids which govern their own transfer by cell-to-cell contact are called conjugative • Tra region: a set of genes within the plasmid that control the transmissability by conjugation • Hfr (high frequency of recombination): strains of bacteria that transfer large amounts of chromosomal DNA during conjugation • Supercoil: plasmids isolated from the cells are in supercoiled configuration • Plasmid separation: by ultracentrifugation or electrophoresis • Curing of plasmids: elimination of plasmids from host cells by various treatments