Preface 合petipddtihutiomntyeas 4 24 Scaffold proteins (AKAPS and others)and their 4 regulatory roles (Chapter 12) ane25esandonoaognsreconmbnatioa Reactive oxygen species as byproducts and as signals (Chapter 19) apgrgnotcRApotnense 4 Structure and function of the oxvgen-evolving Mutation-resistant nature of the genetic code metal cluster in PSII(Chapter 19) (Chapter 27) 4 Insig in cholesterol regulation (Chapter 21) DNA looping,combinatorial control,chromatin 4 S)p Integration of carbohydrate and lipid metabolism Creatine phosphate and the role of creatine kinase in moving ATP to the cytosol (Chapter 23) Steroid-binding nuclear receptors (Chapter 28) New Biochemical Methods An appreciation of biochemistry often requires an under Moder genomic methods (Chapter 9) edition are y (PET)to (Chapter 23) Development of bacterial strains with altered genetic codes for site-specific insertion of novel amino acids into proteins (Chapter 27) rate code"(Chanter 7 New Medical Applications 国 rters and their 么indea or amdent to learn boer Ne sion to colorectal cance th and about the molecular mechanisms of disease ular disease atherosclerosis (Chapter 21) Box 4-6,Death by Misfolding:The Prion Diseases P-450 and drug interactions (Chapter 21) Paganini and Ehlers-Danlos syndrome (Chapter 4) 4 HIV prote nd ho Statins by Inhibitin agulation cascade and hemophilia Chapter6 4 eatment of ess with an enzymati terial suicide inhibitor (Chapter 6) on c profloxacin(the antibiotic effective for anthrax) Stem cells (Chapter 28) viii Preface u Asymmetric lipid distribution in bilayers (Chapter 11) u Role of BAR superfamily proteins in membrane curvature (Chapter 11) u Scaffold proteins (AKAPS and others) and their regulatory roles (Chapter 12) u Reactive oxygen species as byproducts and as signals (Chapter 19) u Structure and function of the oxygen-evolving metal cluster in PSII (Chapter 19) u Formation and transport of lipoproteins in mammals, including the roles of SREBP SCAP, and Insig in cholesterol regulation (Chapter 21) u Integration of carbohydrate and lipid metabolism by PPARs, SREBPs, mTORC1, and LXR (Chapters 21, 23) u Creatine phosphate and the role of creatine kinase in moving ATP to the cytosol (Chapter 23) u Microbial symbionts in the gut and their influence on energy metabolism and adipogenesis (Chapter 23) u Nucleosomes: their modification and positioning and higher-order chromatin structure (Chapter 24) u DNA polymerases and homologous recombination (Chapter 25) u Loading of eukaryotic RNA polymerase II (Chapter 26) u Mutation-resistant nature of the genetic code (Chapter 27) u Regulation of eukaryotic gene expression by miRNAs (Chapters 26 and 28). u DNA looping, combinatorial control, chromatin remodeling, and positive regulation in eukaryotes (Chapter 28) u Regulation of the initiation of transcription in eukaryotes (Chapter 28) u Steroid-binding nuclear receptors (Chapter 28) New Biochemical Methods An appreciation of biochemistry often requires an under￾standing of how biochemical information is obtained. Some of the new methods or updates described in this edition are: u Modern Sanger protein sequencing and mass spectrometry (Chapter 3) u Mass spectrometry applied to proteomics, glycomics, lipidomics, and metabolomics (Chapters 3, 7, 10) u Oligosaccharide microarrays to explore protein￾oligosaccharide interactions and the “carbohydrate code” (Chapter 7) u Modern genomic methods (Chapter 9) u Genetic engineering of photosynthetic organisms (Chapter 20) u Use of positron emission tomography (PET) to visualize tumors and brown adipose tissue (Chapter 23) u Development of bacterial strains with altered genetic codes for site-specific insertion of novel amino acids into proteins (Chapter 27) New Medical Applications This icon is used throughout the book to denote material of special medical interest. As teach￾ers, our goal is for students to learn biochemistry and to understand its relevance to a healthier life and a healthier planet. Many sections explore what we know about the molecular mechanisms of disease. A few of the new or revised medical applications in this edi￾tion are: u Box 4-6, Death by Misfolding: The Prion Diseases u Paganini and Ehlers-Danlos syndrome (Chapter 4) u HIV protease inhibitors and how basic enzymatic principles influenced their design (Chapter 6) u Blood coagulation cascade and hemophilia (Chapter 6) u Curing African sleeping sickness with an enzymatic suicide inhibitor (Chapter 6) u How researchers locate human genes involved in inherited diseases (Chapter 9) u Multidrug resistance transporters and their importance in clinical medicine (Chapter 11) u Multistep progression to colorectal cancer (Chapter 12) u Cholesterol metabolism, cardiovascular disease, and mechanism of plaque formation in atherosclerosis (Chapter 21) u P-450 and drug interactions (Chapter 21) u HMG-CoA reductase (Chapter 21) and Box 21–3, The Lipid Hypothesis and the Development of Statins u Box 24–1, Curing Disease by Inhibiting Topoisomerases, describing the use of topoisomerase inhibitors in the treatment of bacterial infections and cancer, including material on ciprofloxacin (the antibiotic effective for anthrax) u Stem cells (Chapter 28) FMTOC.indd Page viii 09/10/12 1:57 PM user-F408 /Users/user-F408/Desktop