利用蛋白质序列的预测方法 页码,3/20 微酶、一个 reti nobl stoma结合蛋白、肌动蛋白结合蛋白 radi xin和推测是一种GTP酶靶蛋白 的RaBP1等蛋白相似。既然这些蛋白的功能各自不同,它们并不都是想要的结果;然而,其 中许多是DNA的结合蛋白,这就暗示一种可能是在不同功能区中都采用了的十分相似的结构 域。至少进行一次 BLASTP搜索对确认结果和识别关键性残基是十分必要的。 Fragment search: OFF(POS1 and POS2 are begi n and end of sequence) Rank ID DIST LEN2 POS1 POS2 pl DE >p1s181930.00727 1. 33 autoanti gen NOR-90-human ubf1 human1.36‖764 764 5.62 NUCLEOLAR TRANSCRI PTI ON FACTOR 1(UPSTREA ubf1 mouse‖1.40765 7655.55 NUCLEOLAR TRANSCRI PTI ON FACTOR 1(UPSTRE ubf1_rat.57764 17645. 61 NUCLEOLAR TRANSCRI PTI ON FACTOR 1(UPSTREA 456 ubf1 xenl a 3. 95 677 16775.79 NUCLEOLAR TRANSCRI PTI ON FACTOR 1(UPSTREA ubf2 xenl a4.18‖701 17016.05 NUCLEOLAR TRANSCRI PTION FACTOR 2(UPSTREA >p1:s5527.72606 6066.63 hypotheti cal protein YPRO18w-yeast(Sa >p1: i50463849 17725. 71 protei n ki nase -chi cken p1: h540248 83 768 17685. 27 protei n ki nase(EC 2. 1. 37) cdc2-rel atec p1:b540248.87777 7775. 27 protei n ki nase(EC 2. 7.1.37) cdc2-rel ated p1:g540248.9076 17665. 21 protein ki nase (EC 2.7.1.37)cdc2-rel ated 12 >p1:a558179.00‖783 17835. 19 cycl i n-dependent kinase 130-PI TSLRE-mc >p1540491177 7775. 30 protein ki nase(EC 2. 7.1.37)cdc2-rel ated >p1: e54024911779 7795. 42 protei n ki nase(EC 2. 7. 1. 37)cdc2-rel atec 15984. 78HYPOTHETI CAL 69.5 KD PROTEI N C22G705 IN 1:562449‖9 1. 78 hypothetical protein SPAC22G705 >f1:i58390‖9.45‖920 9205.00 reti nobl stoma bi ndi ng protein 1 isoform >p1: $631939.58590 5906 15 hypotheti cal protein YNL227c- yeast(S 19 ynw7-yeast9.58590 15906. 15HYPOTHETI CAL 68.8 KD PROTEIN IN URE2-SSU >p1:s496349.74‖899 899 4.79 hypothetical protein YML093W-yeast(Sa ymj3_yeast 9. 74899 18994 79 HYPOTHETI CAL 103. 0 KD PROTEIN IN RAD10-PF adi_human 9. 76 583 1583‖6.33‖ RADI N radi_pig9.81‖583 5836. RADI XIN(MOESIN B) >f1;i7883‖9.83‖866 18664. 77 reti nobl astoma bi ndi ng protein 1 i soform file://E:wcb生物信息学(中译本)\第十一章利用蛋白质序列的预测方 2005-1-18▔䝊ǃϔϾretinoblastoma㒧ড়㲟ⱑǃ㙠ࡼ㲟ⱑ㒧ড়㲟ⱑradixin੠᥼⌟ᰃϔ⾡GTP䝊䵊㲟ⱑ ⱘRalBP1ㄝ㲟ⱑⳌԐDŽ᮶✊䖭ѯ㲟ⱑⱘࡳ㛑৘㞾ϡৠˈᅗӀᑊϡ䛑ᰃᛇ㽕ⱘ㒧ᵰ˗✊㗠ˈ݊ Ё䆌໮ᰃDNAⱘ㒧ড়㲟ⱑˈ䖭ህᱫ⼎ϔ⾡ৃ㛑ᰃ೼ϡৠࡳ㛑ऎЁ䛑䞛⫼њⱘकߚⳌԐⱘ㒧ᵘ ඳDŽ㟇ᇥ䖯㸠ϔ⃵BLASTP᧰㋶ᇍ⹂䅸㒧ᵰ੠䆚߿݇䬂ᗻ⅟෎ᰃकߚᖙ㽕ⱘDŽ Fragment search: OFF (POS1 and POS2 are begin and end of sequence) Rank ID DIST LEN2 POS1 POS2 pI DE 1 >p1;s18193 0.00 727 1 727 5.33 autoantigen NOR-90 – human 2 ubf1_human 1.36 764 1 764 5.62 NUCLEOLAR TRANSCRIPTION FACTOR 1 (UPSTREAM BINDING FACTOR 1) (UBF 3 ubf1_mouse 1.40 765 1 765 5.55 NUCLEOLAR TRANSCRIPTION FACTOR 1 (UPSTREAM BINDING FACTOR 1) (UBF 4 ubf1_rat 1.57 764 1 764 5.61 NUCLEOLAR TRANSCRIPTION FACTOR 1 (UPSTREAM BINDING FACTOR 1) (UBF 5 ubf1_xenla 3.95 677 1 677 5.79 NUCLEOLAR TRANSCRIPTION FACTOR 1 (UPSTREAM BINDING FACTOR 1) (UBF 6 ubf2_xenla 4.18 701 1 701 6.05 NUCLEOLAR TRANSCRIPTION FACTOR 2 (UPSTREAM BINDING FACTOR 2) (UBF 7 >p1;s57552 7.72 606 1 606 6.63 hypothetical protein YPR018w – yeast (Saccharomyces cerevisiae) 8 >p1;i50463 8.49 772 1 772 5.71 protein kinase – chicken 9 >p1;h54024 8.83 768 1 768 5.27 protein kinase (EC 2.7.1.37) cdc2-related PITSLRE alpha 2 10 >p1;b54024 8.87 777 1 777 5.27 protein kinase (EC 2.7.1.37) cdc2-related PITSLRE alpha 2 11 >p1;g54024 8.90 766 1 766 5.21 protein kinase (EC 2.7.1.37) cdc2-related PITSLRE beta 2 12 >p1;a55817 9.00 783 1 783 5.19 cyclin-dependent kinase 130-PITSLRE – mouse 13 >p1;f54024 9.11 777 1 777 5.30 protein kinase (EC 2.7.1.37) cdc2-related PITSLRE beta 2 14 >p1;e54024 9.11 779 1 779 5.42 protein kinase (EC 2.7.1.37) cdc2-related PITSLRE alpha 2 15 yaa5_schpo 9.45 598 1 598 4.78 HYPOTHETICAL 69.5 KD PROTEIN C22G7.05 IN CHROMOSOME I. 16 >p1;s62449 9.45 598 1 598 4.78 hypothetical protein SPAC22G7.05 – fission yeast (Schizosaccharomyces pombe) 17 >f1;i58390 9.45 920 1 920 5.00 retinoblastoma binding protein 1 isoform I 18 >p1;s63193 9.58 590 1 590 6.15 hypothetical protein YNL227c – yeast (Saccharomyces cerevisiae) 19 ynw7_yeast 9.58 590 1 590 6.15 HYPOTHETICAL 68.8 KD PROTEIN IN URE2-SSU72 INTERGENIC REGION. 20 >p1;s49634 9.74 899 1 899 4.79 hypothetical protein YML093w – yeast (Saccharomyces cerevisiae) 21 ymj3_yeast 9.74 899 1 899 4.79 HYPOTHETICAL 103.0 KD PROTEIN IN RAD10-PRS4 INTERGENIC REGION. 22 radi_human 9.76 583 1 583 6.33 RADIXIN. 23 radi_pig 9.81 583 1 583 6.21 RADIXIN (MOESIN B). 24 >f1;i78883 9.83 866 1 866 4.77 retinoblastoma binding protein 1 isoform II ㄀कϔゴ߽⫼㲟ⱑ䋼ᑣ߫ⱘ乘⌟ᮍ⊩ 义ⷕˈ3/20 file://E:\wcb\⫳⠽ֵᙃᄺ˄Ё䆥ᴀ˅?㄀कϔゴ߽⫼㲟ⱑ䋼ᑣ߫ⱘ乘⌟ᮍ... 2005-1-18 Click to buy NOW! PDF-XCHANGE www.docu-track.com Click to buy NOW! PDF-XCHANGE www.docu-track.com