核技术 第26卷 界上最小、最精密的“马达”更美丽的一面。 999,6:809-811 “基于同步辐射的结构生物学”之所以有这样4 Thompson A C. Vaughan D, Lindau I,eta,x- ray data booklet 大的发展和美好前景,一个关键点是涉及遗传工程、5 Information about the Nobel Prize in Chem19,heod 低温结晶、高灵敏的探测技术、数据处理的计算机硬 SwedishAcademyofSciences(http://www.nobelse) 件和软件等技术上的飞速进步和第三代同步辐射装6 Information about the Nobel Prize in Physiology or Medicine1953 置的出现。基于同步辐射X射线优异的特性,还能 The Royal Swedish Academy of Sciences 在非结晶的状态利用X射线吸收精细结构谱获悉7 ehninger A L, Nelson D L, Cox MM. Principles of biochem 生物大分子中金属活性中心附近的详细特征;用小 8 Yoshida M, Muneyuki E, Hisabori T. Nature Molecular Cell Biology 角散射法探测溶液中大分子的动态和静态大小及形 Reviews,2001,2:669-676 状、追踪蛋白质折叠过程;X射线显微术则提供了一9 Paul d. Boyer Annu Rev Biochem,1997,66:717-749 种新的细胞及细胞器活体的显微成像技术。可以预10 Mitchell F. Physiol Rev,1966,41:45-502 料,同步辐射光源在推动结构生物学和其它生命科 aul D. oyer Biochimica et Biophysica Acta,19.1015 学的发展中将继续发挥不可估量的作用。 12 Abrahams J P, Lutter R, Walker J E, et al. EMBO J, 1993, 12 在本文成文之际,欣闻2002年诺贝尔奖揭晓。 本年度的化学奖授予了在生物大分子结构测定领域13 Information about the Nobel Prize in Chemistry1997(prsm 作出突出贡献的三位科学家 John B Fenn、 Koichi lease), The Royal Swedish Academy of Sciences Tanaka和 Kurt Wuthrich(2),其中 Kurt wuthrich因为15石晓冰,沈允钢生命的化学,19,25-9 对“利用核磁共振技术测定溶液中生物大分子三维 SHI Xiaobing, SHEN Yungang. Chemistry of Life, 1999, 19 结构的方法”作出的突出贡献而获奖,而另两位科 225-229 学家 John B Fenn和 Koichi Tanaka则因为分别发明16翟中和,王喜忠,丁明孝细胞生物学.北京:高等教育出版 了“对生物大分子进行确认和结构分析的方法”和 社,2000.215-221 “对生物大分子的质谱分析的解析技术”而获奖。 ZHAI Zhonghe, WANG Xizhong, DING Mingxiao. Cell Biology Beijing: Higher Educations Press, 2000. 215-2 作为研究生物大分子三维结构的最有力的方法之17 Schultz B E,hmsL. Annu rey biophys Biomol stru,201 一,同步辐射必将与这几种研究方法一起在已经来 30:23-65 临的后基因组时代蛋白质功能与结构的研究中发挥18 Nakamoto R K, Ketchum C J,A- shawi M K, Annu Rev Biophys 更大的作用。 Biomol Struct. 1999. 28: 205--234 19 Noji H, Yasuda R, Yoshida M, et al. Nature, 1997, 386: 299- 参考文献 20 Ealick S E. Nature Struct Biol, 1998, 5: 620-622 Abrahams JP, Leslie A G, Lutter R, et al. Nature, 1994, 370: 21 Yasuda R, Noji H, Yoshida M, et al.Nature,2001, 410:898- 2 Hasnain SS, Helliwell JR, Kamitsubo H. J Synchrotron Rad 2 Information about the Nobel Prize in Chemistry 2002( press re 1998,5:1323 lease), The Royal Swedish Academy of Sciences 3 Hasnain SS, Helliwell J R, Kamitsubo H. J Synchrotron Rad Exploring the source of life energy a research of synchrotron radiation on ATP synthase TIAN Liang ZHANG Xinyi (Synchrotron Radiation Research Center, Fudan University, Shanghai 200433 Abstract The award of a share of the 1997 Nobel Chemistry Prize was the crystal structure of mitochondrial F-ATPase determined at 0. 28nm atomic resolution using data collected at the SRS, Daresbury, UK, and this was the first No- bel Prize for synchrotron radiation-based work. The coming of age of post-genomics was further enforced by syn chrotron radiation with characteristics of high intensity, high collimation and continuous tunable wavelength Key words Synchrotron radiation, Bio-macromolecules, ATP synthase, Three-dimensional structure CLC Q718 ~ it ~26~ * !Jt- -.t:m:/j\ 3l~Wa9" #;~" J!~BB a9 00 0 "£f~~.Ma9~~~~~"~~~~~~ *a9~Ji~~:!1f-ru-~ ~3Cm,~~~lkJft1~IW, 1f£rl!~ ~,~~f!tta9~r9!tlit*,~W~~a9it.m~£ #~~#~it*-.ta9~~*~~~=~~~.M~ .a9 ili £f~~.MxM~~~a9~tl,~ft tE~~~~a9~~5flj ffl x M~I!.&t&~£IB~~i~t1t~ !\m*7t-=f9:t3t:M m'/i 9:t JL' ~JlI a9i$£lBt~{iE; ffllj\ :ft1ttM~~i9!tlm¥l9:t*5t-=fa9Z9J~~D~*/j\])t~ ~,i1§.~mB aJjJT1i:tt~;x M~~~*!nU~ ~T ~ ~~~£IB~lk£IB~Bm~a9~.~.it*o~~~ *4, ~~.~t7'6~tEmZ9J~~ ~~~~t; ~1fPf4 ~~~Ji9:t~~~~~~~~.~~ffl o tE*x~x~~, frklJfJ 2002 !ipit14 $~m ~o *!ip~~~~~~~TtE~~*5t-=f~~.~~~ ~ili*ili:Vi~a9 1irf4~~ John B Fenn,Koichi Tanaka Kurt Wtithrich(22 ) ,~9:t Kurt WUthrich 1ZSJ1-J ~"~ffl~.~~it*ru~m¥l9:t~~*5t-=f . ~fig a9jJ~" ili a9 * ill :Vi iW\ 1i'ff gjt , 1i'ff:1J WHir f-I­ ~* John B Fenn ~ Koichi Tanaka !nU IZSJ 1-J5tJjrj2tw.I T"xt~!\m*5t-=f*fr1ijij~~~~5t;fJTa9jJrt:" ~ "xt~~*5t-=f a9 mtittf5t;fJTIWffJlf;fJTit* " 1i'ffgjt~ o ~1-J~~~!\m*5tT *~~a9:m:~na9jJ~~ ,~?P.M16\~l=:j~J1~~~jJrt:~®tE B ~* ~J§£IZSJ £llst1-tm BmtJ)jfi~l=:j~fig a91iJf~ 9:t ~~ J!*~~ffl o ~ ~ 3t ~ Abrahams J P, Leslie A G, Lutter R, et al. Nature, 1994 , 370: 621-628 2 Hasnain S S , Helliwell J R, Kamitsuho H. Synchrotron Rad, 1998, 5: 1323 3 Hasnain S S, Helliwell J R, Kamitsubo H. Synchrotron Rad, 1999, 6 :809-811 4 Thompson A C, Vaughan D, Lindau I, et al. X - ray data booklet ( 2nd edition) I Lawrence Berkeley National Laboratory, 2001 5 Information about the Nobel Prize in Chemistry 1957 , The Royal Swedish Academy of Sciences. ( http: //www.nobel.se ) 6 Information about the Nobel Prize in Physiology or Medicine 1953, The Royal Swedish Academy of Sciences 7 Lehninger A L, Nelson D L, Cox M M. Principles of biochemistry ( 2nd edition) , New York Worth publishers, 1993 8 Yoshida M, Muneyuki E, Hisabori T. Nature Molecular Cell Biology Reviews, 2001 , 2 :669-676 9 Paul D. Boyer Annu Rev Biochem, 1997, 66:7 17 - 749 10 Mitchell P. Physiol Rev, 1966, 41 :445- 502 11 Paul D. Boyer Biochimica et Biophysica Acta, 1993,1140:215­ 250 12 Abrahams J P, Lutter R , Walker J E, et al. EMBO J, 1993, 12: 1775- 1780 13 Information about the Nobel Prize in Chemistry 1997 ( press re￾lease) , The Royal Swedish Academy of Sciences 14 Wang H Y, Oster G. Nature, 1998, 396 :279 15 :fiiJ1Et;j(, ttftm. ~-$8{J1t~, 1999·, 19 :225-229 SHI Xiaobing, SHEN Yungang. Chemistry of Life, 1999, 19: 225-229 16 ilrJ:tln, £~ ~" T~~. ~JfflEE4&J~. :ftJi(:~~¥i1flliJtli fi, 2000. 215-221 ZHAI Zhonghe, WANG Xizhong, DING Mingxiao. Cell Biology. Beijing: Higher Educations Press, 2000. 215-221 17 Schultz BE, Chan S I. Annu Rev Biophys Biomol Struct, 200 1 , 30:23-65 18 Nakamoto R K, Ketchum C J, AI - shawi M K. Annu Rev Biophys Biomol Struct, 1999, 28: 205- 234 19 Noji H , Yasuda R, Yoshida M, et al. Nature, 1997, 386: 299­ 302 20 Ealick S E. Nature Struct BioI, 1998, 5 :620-622 21 Yasuda R, Noji H, Yoshida M, et al. Nature, 2001,410:898­ 904 22 Information about the Nobel Prize in Chemistry 2002 ( press re￾lease ) , The Royal Swedish Academy of Sciences Exploring the source of life energy --A research of synchrotron radiation on ATP synthase TIAN Liang ZHANG Xinyi ( Synchrotron Radiation Research Center, Fudan University, Shanghai 200433 ) Abstract The award of a share of the 1997 Nobel Chemistry Prize was the crystal structure of mitochondrial Fl - ATPase determined at O. 28nm atomic resolution using data collected at the SRS, Daresbury, UK, and this was the first No￾bel Prize for synchrotron radiation - based work. The coming of age of post - genomics was further enforced by syn￾chrotron radiation with characteristics of high intensity, high collimation and continuous tunable wavelength. Key words Synchrotron radiation, Bio - macromolecules , ATP synthase, Three - dimensional structure CLC Q71