高通量DNA测序技术 Emulsion PCR (emPCR) a Roche/454, Life/APG, Polonator Emulsion PCR One DNA molecule per bead. Clonal amplification to thousands of copies occurs in microreactors in an emulsion 100-200 million beads PCR dissociation Primer, template, Chemically cross- dNTPs and polymerase linked to a glass slide Nature Rey genet 2010. 11: 31Emulsion PCR (emPCR) Nature Rev Genet 2010, 11:31 高通量DNA测序技术